Back to Search
Start Over
Therapeutic effects of astragaloside IV on myocardial injuries: multi-target identification and network analysis
- Source :
- PLoS ONE, PLoS ONE, Vol 7, Iss 9, p e44938 (2012)
- Publication Year :
- 2012
-
Abstract
- Astragaloside IV (AGS-IV) is a main active ingredient of Astragalus membranaceus Bunge, a medicinal herb used for cardiovascular diseases (CVD). In this work, we investigated the therapeutic mechanisms of AGS-IV at a network level by computer-assisted target identification with the in silico inverse docking program (INVDOCK). Targets included in the analysis covered all signaling pathways thought to be implicated in the therapeutic actions of all CVD drugs approved by US FDA. A total of 39 putative targets were identified. Three of these targets, calcineurin (CN), angiotensin-converting enzyme (ACE), and c-Jun N-terminal kinase (JNK), were experimentally validated at a molecular level. Protective effects of AGS-IV were also compared with the CN inhibitor cyclosporin A (CsA) in cultured cardiomyocytes exposed to adriamycin. Network analysis of protein-protein interactions (PPI) was carried out with reference to the therapeutic profiles of approved CVD drugs. The results suggested that the therapeutic effects of AGS-IV are based upon a combination of blocking calcium influx, vasodilation, anti-thrombosis, anti-oxidation, anti-inflammation and immune regulation.
- Subjects :
- Protein Conformation
lcsh:Medicine
Pharmacology
Cardiovascular
Biophysics Simulations
0302 clinical medicine
Cyclosporin a
Protein Interaction Mapping
Biochemical Simulations
Myocytes, Cardiac
Molecular Targeted Therapy
Biomacromolecule-Ligand Interactions
lcsh:Science
Drug Approval
0303 health sciences
Multidisciplinary
Kinase
Drug discovery
Systems Biology
3. Good health
Molecular Docking Simulation
Cardiovascular Diseases
030220 oncology & carcinogenesis
Medicine
Signal transduction
medicine.drug
Signal Transduction
Research Article
Biophysics
Cardiovascular Pharmacology
03 medical and health sciences
Complementary and Alternative Medicine
medicine
Humans
Doxorubicin
Biology
030304 developmental biology
Binding Sites
business.industry
United States Food and Drug Administration
Therapeutic effect
lcsh:R
Computational Biology
Reproducibility of Results
Saponins
Triterpenes
United States
Calcineurin
Docking (molecular)
Cytoprotection
lcsh:Q
business
Subjects
Details
- ISSN :
- 19326203
- Volume :
- 7
- Issue :
- 9
- Database :
- OpenAIRE
- Journal :
- PloS one
- Accession number :
- edsair.doi.dedup.....78d86eebd07bd9e5c5e50bb03c8a5ece