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MALDI-TOF MS analysis of human and animal Brachyspira species and benefits of database extension
- Source :
- Journal of Proteomics. 78:273-280
- Publication Year :
- 2013
- Publisher :
- Elsevier BV, 2013.
-
Abstract
- Spirochaetes belonging to the genus Brachyspira are anaerobic bacteria that colonize the large intestine of humans and animals, mainly pigs. The main species are namely, B. hyodysenteriae, the etiological agent of swine dysentery, B. pilosicoli, a zoonotic agent causing colonic spirochaetosis both in humans and different animal species, B. aalborgi, exclusively infecting humans causing colonic spirochaetosis, B. intermedia, a potential animal pathogen, B. innocens and B. murdochii, generally commensal of pigs, and B. alvinipulli, found in egg laying hens with diarrhea. In this study, for the first time, MALDI-TOF MS was applied on Brachyspira strains of human and animal origins, supplementing the existing database, limited to the species B. murdochii only, with spirochaetal protein profiles and demonstrating its usefulness in the rapid, cheap and reliable identification of Brachyspira strains at the species level, overcoming the problems previously encountered in the identification of these spirochaetes when using biochemical and genetic-based methods. Moreover, a dendrogram based on protein profiles of the different spirochaetal species was generated reflecting their host spectrum, showing in the same branch the only two species able to infect humans (B. aalborgi and B. pilosicoli) and in the other branch the spirochaetes infecting exclusively animals.
- Subjects :
- Brachyspira
Swine
Biophysics
Biology
computer.software_genre
Biochemistry
Genus Brachyspira
Microbiology
Bacterial Proteins
Species Specificity
medicine
Animals
Humans
Databases, Protein
Pathogen
Database
Host (biology)
Brachyspira species
biology.organism_classification
Diarrhea
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
Spirochaete
Anaerobic bacteria
medicine.symptom
Gram-Negative Bacterial Infections
computer
Subjects
Details
- ISSN :
- 18743919
- Volume :
- 78
- Database :
- OpenAIRE
- Journal :
- Journal of Proteomics
- Accession number :
- edsair.doi.dedup.....78d2c29504cca909695b50e45fd81909