Additional file 7 of Mitophagy promotes sorafenib resistance through hypoxia-inducible ATAD3A dependent Axis

Additional file 7: Supplementary Fig. 4. ATAD3A is down-regulated in hypoxia and a potential target of miR-210-5P. a Immunofluorescent staining of HIF-1α (green) and ATAD3A (red) expression in normoxic and hypoxic LM3 cells (1% O2 treatment for 24 h). Scale bar = 200 μm. b Immunoblot showing expression levels of indicated proteins in Huh7 cells with/without CoCl2 treatment for different durations. c qRT-PCR analysis of ATAD3A expression in LM3 cells with/without CoCl2 treatment. d Bioinformatics analysis of the hypoxia-responsive miRNAs from digestive cancers. e qRT-PCR analysis showing miR-210-5P levels in Huh7 and LM3 cells treated with DMSO or CoCl2 for 24 h. f qRT-PCR analysis showing the expression of ATAD3A in LM3 cells treated with miR-210-5P mimic and inhibitor. Each bar represents the mean ± SEM of three independent experiments. *P