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Functional analysis of all aminotransferase proteins inferred from the genome sequence of Corynebacterium glutamicum
- Source :
- Journal of bacteriology 187, 7639-7646 (2005). doi:10.1128/JB.187.22.7639-7646.2005
- Publication Year :
- 2005
- Publisher :
- Soc., 2005.
-
Abstract
- Twenty putative aminotransferase (AT) proteins of Corynebacterium glutamicum , or rather pyridoxal-5′-phosphate (PLP)-dependent enzymes, were isolated and assayed among others with l -glutamate, l -aspartate, and l -alanine as amino donors and a number of 2-oxo-acids as amino acceptors. One outstanding AT identified is AlaT, which has a broad amino donor specificity utilizing (in the order of preference) l -glutamate > 2-aminobutyrate > l -aspartate with pyruvate as acceptor. Another AT is AvtA, which utilizes l -alanine to aminate 2-oxo-isovalerate, the l -valine precursor, and 2-oxo-butyrate. A second AT active with the l -valine precursor and that of the other two branched-chain amino acids, too, is IlvE, and both enzyme activities overlap partially in vivo, as demonstrated by the analysis of deletion mutants. Also identified was AroT, the aromatic AT, and this and IlvE were shown to have comparable activities with phenylpyruvate, thus demonstrating the relevance of both ATs for l -phenylalanine synthesis. We also assessed the activity of two PLP-containing cysteine desulfurases, supplying a persulfide intermediate. One of them is SufS, which assists in the sulfur transfer pathway for the Fe-S cluster assembly. Together with the identification of further ATs and the additional analysis of deletion mutants, this results in an overview of the ATs within an organism that may not have been achieved thus far.
- Subjects :
- metabolism [Pyruvic Acid]
Phenylpyruvic Acids
Corynebacterium glutamicum
Substrate Specificity
metabolism [Butyric Acids]
Aspartic acid
Pyruvic Acid
genetics [Transaminases]
metabolism [Carbon-Sulfur Lyases]
chemistry.chemical_classification
Alanine
enzymology [Corynebacterium glutamicum]
Nucleic acid sequence
alpha-ketoisovalerate
Keto Acids
Amino acid
Butyrates
Carbon-Sulfur Lyases
Biochemistry
metabolism [Keto Acids]
genetics [Bacterial Proteins]
metabolism [Bacterial Proteins]
alpha-keto-beta-methylvaleric acid
Butyric Acids
Glutamic Acid
Biology
Microbiology
cysteine desulfurase
Hemiterpenes
alpha-ketobutyric acid
Bacterial Proteins
ddc:570
Molecular Biology
metabolism [Alanine]
Transaminases
isolation & purification [Transaminases]
Aspartic Acid
isolation & purification [Bacterial Proteins]
metabolism [Glutamic Acid]
genetics [Corynebacterium glutamicum]
Glutamic acid
Enzymes and Proteins
metabolism [Aspartic Acid]
Enzyme
phenylpyruvic acid
chemistry
alpha-ketoisocaproic acid
metabolism [Phenylpyruvic Acids]
metabolism [Transaminases]
Gene Deletion
Cysteine
Subjects
Details
- Language :
- English
- Database :
- OpenAIRE
- Journal :
- Journal of bacteriology 187, 7639-7646 (2005). doi:10.1128/JB.187.22.7639-7646.2005
- Accession number :
- edsair.doi.dedup.....78cb1940b01ec3bd7e55b98e457837c9