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Apoptotic effect of MG-132 on human tongue squamous cell carcinoma
- Source :
- Biomedicine & Pharmacotherapy. 65:322-327
- Publication Year :
- 2011
- Publisher :
- Elsevier BV, 2011.
-
Abstract
- The aim of this study was to investigate the apoptotic effect of a proteasome inhibitor MG-132 on Tca-8113, a cell line of human tongue squamous cell carcinoma. Tca-8113 cells were treated with 10, 20, and 30μM of MG-132, or 5μM thapsigargin. Apoptosis rate was determined with annexin V/propidium iodide double staining. Expression of E3ubiquitin-protein ligase was determined by ELISA, and Grp78 and caspase-12 mRNA, and Grp78 and caspase-12 protein was assessed by RT-PCR and Western blot, respectively. Apoptosis was observed 18h after MG-132 treatment. The apoptotic rate in the 10, 20, and 30μM MG-132 group was 13.5, 19.6 and 34.7%, respectively, which was higher than in the thapsigargin (8.5%, P
- Subjects :
- Thapsigargin
Leupeptins
Ubiquitin-Protein Ligases
Antineoplastic Agents
Apoptosis
Cysteine Proteinase Inhibitors
Biology
chemistry.chemical_compound
Downregulation and upregulation
Western blot
Annexin
Cell Line, Tumor
medicine
Humans
RNA, Messenger
Propidium iodide
Endoplasmic Reticulum Chaperone BiP
Caspase 12
Heat-Shock Proteins
Pharmacology
medicine.diagnostic_test
General Medicine
Molecular biology
Tongue Neoplasms
Ubiquitin ligase
chemistry
Carcinoma, Squamous Cell
Proteasome inhibitor
biology.protein
medicine.drug
Subjects
Details
- ISSN :
- 07533322
- Volume :
- 65
- Database :
- OpenAIRE
- Journal :
- Biomedicine & Pharmacotherapy
- Accession number :
- edsair.doi.dedup.....78b08124364aac291c0affe1fba075e4