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A study of immunomodulatory genes responses to macrophages of Schistosoma japonicum infection during different stages by microarray analysis
- Source :
- Acta tropica. 127(3)
- Publication Year :
- 2011
-
Abstract
- Macrophages initiate, modulate, and also serve as final effector cells in immune responses during the course of schistosomal infections. In this study, we investigated the gene expression profile and functional changes of macrophages in immune responses against the Schistosoma japonicum by microarray analysis. Hierarchical clustering analysis demonstrated that a significant switch in gene transformation associated with a type-1 response and linked with a type-2 cytokine phenotype occurs between 4.5 and 8 weeks post-infection. Moreover, the gene profiles at 3 later time-points following egg challenge were similar in complexity and magnitude. The data also showed that there were mostly inhibition of gene expression related TLR, IFN, MHC and TNFrsf at the switch between 4.5 and 8 weeks post-infection, It is suggested that these immunomodulatory genes may be down-regulated in defense against S. japonicum eggs and granuloma pathology. The induction of alternatively activated macrophage (AAMϕ) was important for dampening the inflammation in hepatic granulomas and contributing to a decrease in cytotoxicity. The gene expressions involved in repair/remodeling during liver fibrosis were also observed after egg production. Understanding the immune mechanisms associated with parasitic resistance, pathology of parasite infection, and parasite growth will provide useful insight on host-schistosome interactions and for the control of schistosomiasis.
- Subjects :
- Male
Veterinary (miscellaneous)
medicine.medical_treatment
Protein Array Analysis
Inflammation
Schistosoma japonicum
Mice
Immune system
Gene expression
medicine
Macrophage
Animals
Schistosoma Japonicum Infection
biology
Microarray analysis techniques
Macrophages
biology.organism_classification
Flow Cytometry
Mice, Inbred C57BL
Infectious Diseases
Cytokine
Gene Expression Regulation
Insect Science
Schistosomiasis japonica
Immunology
RNA
Parasitology
medicine.symptom
Subjects
Details
- ISSN :
- 18736254
- Volume :
- 127
- Issue :
- 3
- Database :
- OpenAIRE
- Journal :
- Acta tropica
- Accession number :
- edsair.doi.dedup.....788d3d72af82a87cb5ef0f2f8a1917cd