Regulation of matrix metalloproteinases-8,-9 and endogenous tissue inhibitor-1 in oral biofluids during pregnancy and postpartum

Objective: During pregnancy, mothers undergoe considerable physiological changes affecting the whole body including periodontal tissues. Susceptibility to gingival inflammation during pregnancy could be mediated by modulation of matrix metalloproteinases (MMPs) and their tissue inhibitors (TIMPs). Therefore, the aim of this study was to investigate salivary and gingival crevicular fluid (GCF) levels of MMPs and TIMPs during the second and third trimester of pregnancy and postpartum. Design: Saliva and GCF samples were collected from 96 pregnant women (PW) before and after giving birth. The sixty matched non-pregnant women (N-PW) were recruited as a control group and full-mouth periodontal examination was performed. The levels of MMP-8, MMP-9 and TIMP-1 were determined by immunofluorometric and enzyme-linked immunosorbent assays. Results: The PW group exhibited significantly higher levels of MMP-8 and MMP-9 in their saliva than the N-PW group while corresponding salivary TIMP-1 levels were significantly lower in NPW compared to the postpartum stage. This resulted in significantly higher MMP-8/TIMP-1 and MMP-9/TIMP-1ratio in the saliva from PW before and after birth than in that from N-PW. MMP-8, MMP-9 and TIMP-1 levels were higher in GCF from PW and postpartum than in that from N-PW. Conclusions: MMP-8 and MMP-9 levels in saliva and GCF reflect inflammatory burden during pregnancy. They could be used for monitoring the inflammatory state of gingival tissues during pregnancy. © 2021
2292018, 2512017, TYH2512016, Y1149SUL32 Karolinska Institutet, KI: 10488415B2
The research in our laboratories has been supported by grants from the Helsinki University Hospital Research Foundation (TYH2512016, 2512017, 2292018, Y1149SUL32), Apollonia Foundation, Scientific and Technological Research Council of Turkey (T?bitak Grant program 2219) and the strategic funds from Karolinska Institutet, Stockholm, Sweden. Prof Timo Sorsa is an inventor of US-patents 10488415B2 and Japan Patent 2016 -554676.
The research in our laboratories has been supported by grants from the Helsinki University Hospital Research Foundation ( TYH2512016 , 2512017 , 2292018 , Y1149SUL32 ), Apollonia Foundation, Scientific and Technological Research Council of Turkey (Tübitak Grant program 2219 ) and the strategic funds from Karolinska Institutet, Stockholm, Sweden . Prof Timo Sorsa is an inventor of US-patents 10488415B2 and Japan Patent 2016 -554676.