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Dynamic interaction of hTRPC6 with the Orai1–STIM1 complex or hTRPC3 mediates its role in capacitative or non-capacitative Ca2+ entry pathways
- Source :
- Biochemical Journal. 420:267-277
- Publication Year :
- 2009
- Publisher :
- Portland Press Ltd., 2009.
-
Abstract
- TRPC (canonical transient receptor potential) channel subunits have been shown to assemble into homo- or hetero-meric channel complexes, including different Ca 2+ -handling proteins, required for the activation of CCE (capacitative Ca 2+ entry) or NCCE (non-CCE) pathways. In the present study we found evidence for the dynamic interaction between endogenously expressed hTRPC6 (human TRPC6) with either both Orai1 and STIM1 (stromal interaction molecule 1) or hTRPC3 to participate in CCE or NCCE. Electrotransjection of cells with an anti-hTRPC6 antibody, directed towards the C-terminal region, reduces CCE induced by TPEN [ N , N , N ′, N ′-tetrakis-(2-pyridylmethyl)-ethylenediamine], which reduces the intraluminal free Ca 2+ concentration. Cell stimulation with thrombin or extensive Ca 2+ -store depletion by TG (thapsigargin)+ionomycin enhanced the interaction between hTRPC6 and the CCE proteins Orai1 and STIM1. In contrast, stimulation with the diacylglycerol analogue OAG (1-oleoyl-2-acetyl- sn -glycerol) displaces hTRPC6 from Orai1 and STIM1 and enhances the association between hTRPC6 and hTRPC3. The interaction between hTRPC6 and hTRPC3 was abolished by dimethyl-BAPTA [1,2-bis-( o -aminophenoxy)ethane- N , N , N ′, N ′-tetra-acetic acid] loading, which indicates that this phenomenon is Ca 2+ -dependent. These findings support the hypothesis that hTRPC6 participates both in CCE and NCCE through its interaction with the Orai1–STIM1 complex or hTRPC3 respectively.
- Subjects :
- Blood Platelets
Thapsigargin
ORAI1 Protein
Cell Survival
Stereochemistry
Blotting, Western
Biochemistry
TRPC6
Diglycerides
chemistry.chemical_compound
Transient receptor potential channel
TRPC6 Cation Channel
Humans
Immunoprecipitation
Stromal Interaction Molecule 1
Egtazic Acid
Molecular Biology
TRPC
Chelating Agents
TRPC Cation Channels
Diacylglycerol kinase
ORAI1
Ionomycin
Membrane Proteins
STIM1
Cell Biology
Ethylenediamines
Neoplasm Proteins
chemistry
Biophysics
Calcium
Calcium Channels
Protein Binding
Signal Transduction
Subjects
Details
- ISSN :
- 14708728 and 02646021
- Volume :
- 420
- Database :
- OpenAIRE
- Journal :
- Biochemical Journal
- Accession number :
- edsair.doi.dedup.....787b564f009af7a51911404bd8326342