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α-Solanine impairs oocyte maturation and quality by inducing autophagy and apoptosis and changing histone modifications in a pig model
- Source :
- Reproductive toxicology (Elmsford, N.Y.). 75
- Publication Year :
- 2017
-
Abstract
- In this study, we used a pig model to investigate the effects of α-solanine (a natural toxin found mainly in potato sprouts) on oocyte maturation, quality and subsequent embryonic development. We found that α-solanine (10 μM) disturbed meiotic resumption and increased abnormal spindle formation and altered the cortical granule (CG) distribution compared with the untreated group. α-Solanine triggered autophagy and apoptosis by increasing the expressions of autophagy-related genes (LC3, ATG7, and LAMP2) and apoptotic related genes (BAX and CASP3). Exposure of porcine oocytes to α-solanine significantly increased the levels of H3K36me3 and H3K27me3. Moreover, α-solanine significantly reduced the cleavage and blastocyst formation rates, decreased the total and inner cell mass cells numbers, and increased apoptosis in these porcine embryos. Taken together, our data indicate that α-solanine toxically impairs oocyte maturation and quality by triggering autophagy/apoptosis and facilitating epigenetic modifications. Furthermore, α-solanine suppressed subsequent embryonic development and reduced embryo quality.
- Subjects :
- 0301 basic medicine
Cell Survival
Swine
Cortical granule
Gene Expression
Apoptosis
Spindle Apparatus
Toxicology
03 medical and health sciences
medicine
Autophagy
Inner cell mass
Animals
Blastocyst
Cells, Cultured
Membrane Potential, Mitochondrial
Chemistry
Embryogenesis
Oocyte
Cell biology
Histone Code
Solanine
030104 developmental biology
medicine.anatomical_structure
Oocytes
Multipolar spindles
Subjects
Details
- ISSN :
- 18731708
- Volume :
- 75
- Database :
- OpenAIRE
- Journal :
- Reproductive toxicology (Elmsford, N.Y.)
- Accession number :
- edsair.doi.dedup.....7866804a8d710d122dbd86c375093f94