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Metabolic Footprints of Burkholderia Sensu Lato Rhizosphere Bacteria Active against Maize Fusarium Pathogens
- Source :
- Microorganisms, Volume 9, Issue 10, Microorganisms, Vol 9, Iss 2061, p 2061 (2021)
- Publication Year :
- 2021
- Publisher :
- MDPI AG, 2021.
-
Abstract
- Consistent with their reported abundance in soils, several Burkholderia sensu lato strains were isolated from the rhizosphere of maize plants cultivated at different sites in central México. Comparative analysis of their 16S rRNA gene sequences permitted their separation into three distinctive clades, which were further subdivided into six other clusters by their close resemblance to (1) Trinickia dinghuensis<br />(2) Paraburkholderia kirstenboschensis, P. graminis, P. dilworthii and P. rhynchosiae<br />(3) B. gladioli<br />(4) B. arboris<br />(5) B. contaminans, or (6) B. metallica representative species. Direct confrontation assays revealed that these strains inhibited the growth of pathogenic Fusarium oxysporum f. sp. radicis-lycopersici, and F. verticillioides within a roughly 3–55% inhibition range. The use of a DIESI-based non-targeted mass spectroscopy experimental strategy further indicated that this method is an option for rapid determination of the pathogen inhibitory capacity of Burkholderia sensu lato strains based solely on the analysis of their exometabolome. Furthermore, it showed that the highest anti-fungal activity observed in B. contaminans and B. arboris was associated with a distinctive abundance of certain m/z ions, some of which were identified as components of the ornbactin and pyochelin siderophores. These results highlight the chemical diversity of Burkholderia sensu lato bacteria and suggest that their capacity to inhibit the Fusarium-related infection of maize in suppressive soils is associated with siderophore synthesis.
- Subjects :
- Microbiology (medical)
Fusarium
Siderophore
food.ingredient
QH301-705.5
Burkholderia
Microbiology
Article
Paraburkholderia
food
Virology
parasitic diseases
Botany
Fusarium oxysporum
biocontrol
Biology (General)
Rhizosphere
biology
siderophores
bacterial infections and mycoses
biology.organism_classification
16S ribosomal RNA
Bacteria
DIESI-MS
Subjects
Details
- ISSN :
- 20762607
- Volume :
- 9
- Database :
- OpenAIRE
- Journal :
- Microorganisms
- Accession number :
- edsair.doi.dedup.....785532fe0afd6ce063aee473f9ad1151
- Full Text :
- https://doi.org/10.3390/microorganisms9102061