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Metabolic Footprints of Burkholderia Sensu Lato Rhizosphere Bacteria Active against Maize Fusarium Pathogens

Authors :
Guadalupe Coyolxauhqui Barrera-Galicia
José A. Vera-Núñez
Héctor A. Peniche-Pavía
Juan José Peña-Cabriales
John P. Délano-Frier
Sergio A. Covarrubias
Source :
Microorganisms, Volume 9, Issue 10, Microorganisms, Vol 9, Iss 2061, p 2061 (2021)
Publication Year :
2021
Publisher :
MDPI AG, 2021.

Abstract

Consistent with their reported abundance in soils, several Burkholderia sensu lato strains were isolated from the rhizosphere of maize plants cultivated at different sites in central México. Comparative analysis of their 16S rRNA gene sequences permitted their separation into three distinctive clades, which were further subdivided into six other clusters by their close resemblance to (1) Trinickia dinghuensis<br />(2) Paraburkholderia kirstenboschensis, P. graminis, P. dilworthii and P. rhynchosiae<br />(3) B. gladioli<br />(4) B. arboris<br />(5) B. contaminans, or (6) B. metallica representative species. Direct confrontation assays revealed that these strains inhibited the growth of pathogenic Fusarium oxysporum f. sp. radicis-lycopersici, and F. verticillioides within a roughly 3–55% inhibition range. The use of a DIESI-based non-targeted mass spectroscopy experimental strategy further indicated that this method is an option for rapid determination of the pathogen inhibitory capacity of Burkholderia sensu lato strains based solely on the analysis of their exometabolome. Furthermore, it showed that the highest anti-fungal activity observed in B. contaminans and B. arboris was associated with a distinctive abundance of certain m/z ions, some of which were identified as components of the ornbactin and pyochelin siderophores. These results highlight the chemical diversity of Burkholderia sensu lato bacteria and suggest that their capacity to inhibit the Fusarium-related infection of maize in suppressive soils is associated with siderophore synthesis.

Details

ISSN :
20762607
Volume :
9
Database :
OpenAIRE
Journal :
Microorganisms
Accession number :
edsair.doi.dedup.....785532fe0afd6ce063aee473f9ad1151
Full Text :
https://doi.org/10.3390/microorganisms9102061