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SUMO modification selectively regulates transcriptional activity of peroxisome-proliferator-activated receptor γ in C2C12 myotubes
- Source :
- Biochemical Journal. 433:155-161
- Publication Year :
- 2010
- Publisher :
- Portland Press Ltd., 2010.
-
Abstract
- PPAR (peroxisome-proliferator-activated receptor) γ, a nuclear receptor, can be conjugated with SUMO (small ubiquitin-like modifier), which results in the negative regulation of its transcriptional activity. In the present study, we tested whether de-SUMOylation of PPARγ affects the expression of PPARγ target genes in mouse muscle cells and investigated the mechanism by which de-SUMOylation increases PPARγ transcriptional activity. We found that the SUMO-specific protease SENP2 [SUMO1/sentrin/SMT3 (suppressor of mif two 3 homologue 1)-specific peptidase 2] effectively de-SUMOylates PPARγ–SUMO conjugates. Overexpression of SENP2 in C2C12 cells increased the expression of some PPARγ target genes, such as FABP3 (fatty-acid-binding protein 3) and CD36 (fatty acid translocase), both in the absence and presence of rosiglitazone. In contrast, overexpression of SENP2 did not affect the expression of another PPARγ target gene ADRP (adipose differentiation-related protein). De-SUMOylation of PPARγ increased ChIP (chromatin immunoprecipitation) of both a recombinant PPRE (PPAR-response element) and endogenous PPREs of the target genes CD36 and FABP3, but ChIP of the PPRE in the ADRP promoter was not affected by SENP2 overexpression. In conclusion, these results indicate that SENP2 de-SUMOylates PPARγ in myotubes, and de-SUMOylation of PPARγ selectively increases the expression of some PPARγ target genes.
- Subjects :
- CD36 Antigens
Transcriptional Activation
CD36
Muscle Fibers, Skeletal
Peroxisome proliferator-activated receptor
Biology
Fatty Acid-Binding Proteins
Biochemistry
Cell Line
Mice
Multienzyme Complexes
Animals
Receptor
Molecular Biology
chemistry.chemical_classification
Regulation of gene expression
Myogenesis
Sumoylation
Cell Biology
Molecular biology
PPAR gamma
Cysteine Endopeptidases
Gene Expression Regulation
chemistry
Nuclear receptor
Cell culture
biology.protein
Fatty Acid Binding Protein 3
Chromatin immunoprecipitation
Subjects
Details
- ISSN :
- 14708728 and 02646021
- Volume :
- 433
- Database :
- OpenAIRE
- Journal :
- Biochemical Journal
- Accession number :
- edsair.doi.dedup.....78509b23b48ebbde50c3378cd5d00843