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Astroglial Cx30 differentially impacts synaptic activity from hippocampal principal cells and interneurons
- Source :
- Glia, Glia, Wiley, 2021, Online ahead of print. ⟨10.1002/glia.24017⟩, Glia, Wiley, 2021, ⟨10.1002/glia.24017⟩, Glia, Wiley, In press, Glia, 2021, Online ahead of print. ⟨10.1002/glia.24017⟩
- Publication Year :
- 2021
- Publisher :
- HAL CCSD, 2021.
-
Abstract
- International audience; Astrocytes play important roles in brain function via dynamic structural and functional interactions with neurons. Yet the underlying mechanisms remain poorly defined. A typical feature of astrocytes is the high expression of connexins, which mediate their extensive intercellular communication and regulate their structural properties. In particular, connexin 30 (Cx30), one of the two connexins abundantly expressed by astrocytes, was recently shown to be a critical regulator of excitatory synaptic transmission by controlling the astroglial coverage of synapses. However, the role of Cx30 in the regulation of inhibitory synaptic transmission and excitatory/inhibitory balance remains elusive. Here, we investigated the role of astroglial Cx30 on the electrophysiological and morphological properties of five classes of hippocampal CA1 stratum oriens and pyramidale neurons, defined by the unsupervised Ward’s clustering. Using Cx30 knockout mice, we found that Cx30 alters specific properties of some subsets of CA1 interneurons, such as resting membrane potential and sag ratio, while other parameters, such as action potential threshold and saturation frequency, were more frequently altered among the different classes of neurons. The excitation-inhibition balance was also differentially and selectively modulated among the different neuron subtypes. Only slight morphological differences were observed on reconstructed neurons. Altogether, these data indicate that Cx30 differentially alters the electrophysiological and morphological properties of hippocampal cell populations, and modulates both their excitatory and inhibitory inputs. Astrocytes, via Cx30, are thus active modulators of both excitatory and inhibitory synapses in the hippocampus.
- Subjects :
- 0301 basic medicine
Role of astroglial Cx30 on synaptic networks neuroglial interactions
Action potential
hippocampus
[SDV.NEU.NB]Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC]/Neurobiology
Hippocampus
Neurotransmission
Hippocampal formation
Biology
Inhibitory postsynaptic potential
Mice
03 medical and health sciences
Cellular and Molecular Neuroscience
0302 clinical medicine
Interneurons
medicine
excitation-inhibition balance
Animals
synaptic transmission
[SDV.NEU] Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC]
Membrane potential
[SDV.NEU.NB] Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC]/Neurobiology
astrocytes
patch-clamp
connexin 30
030104 developmental biology
medicine.anatomical_structure
Neurology
Synapses
Excitatory postsynaptic potential
[SDV.NEU]Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC]
Neuron
Neuroscience
030217 neurology & neurosurgery
Subjects
Details
- Language :
- English
- ISSN :
- 08941491 and 10981136
- Database :
- OpenAIRE
- Journal :
- Glia, Glia, Wiley, 2021, Online ahead of print. ⟨10.1002/glia.24017⟩, Glia, Wiley, 2021, ⟨10.1002/glia.24017⟩, Glia, Wiley, In press, Glia, 2021, Online ahead of print. ⟨10.1002/glia.24017⟩
- Accession number :
- edsair.doi.dedup.....782503615020760534d07990c8cf81b5