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Impact of intracellular metallothionein on metal biouptake and partitioning dynamics at bacterial interfaces
- Source :
- Physical Chemistry Chemical Physics, Physical Chemistry Chemical Physics, Royal Society of Chemistry, 2017, 19 (43), pp.29114-29124. ⟨10.1039/c7cp05456d⟩
- Publication Year :
- 2017
- Publisher :
- HAL CCSD, 2017.
-
Abstract
- Genetically engineered microorganisms are alternatives to physicochemical methods for remediation of metal-contaminated aquifers due to their remarkable bioaccumulation capacities. The design of such biosystems would benefit from the elaboration of a sound quantitative connection between performance in terms of metal removal from aqueous solution and dynamics of the multiscale processes leading to metal biouptake. In this work, this elaboration is reported for Escherichia coli cells modified to overexpress intracellular metallothionein (MTc), a strong proteinaceous metal chelator. Depletion kinetics of Cd(II) from bulk solution following biouptake and intracellular accumulation is addressed as a function of cell volume fraction using electroanalytical probes and ligand exchange-based analyses. It is shown that metal biouptake in the absence and presence of MTc is successfully interpreted on the basis of a formalism recently developed for metal partitioning dynamics at biointerfaces with integration of intracellular metal speciation. The analysis demonstrates how fast sequestration of metals by intracellular MTc bypasses metal excretion (efflux) and enhances the rate of metal depletion to an extent such that complete removal is achieved at sufficiently large cell volume fractions. The magnitude of the stability constant of nanoparticulate metal–MTc complexes, as derived from refined analysis of macroscopic bulk metal depletion data, is further confirmed by independent electrochemical measurement of metal binding by purified MTc extracts.
- Subjects :
- 0301 basic medicine
Kinetics
Analytical chemistry
General Physics and Astronomy
010501 environmental sciences
01 natural sciences
Metal Chelator
Metal
03 medical and health sciences
[CHIM.ANAL]Chemical Sciences/Analytical chemistry
Escherichia coli
Metallothionein
Physical and Theoretical Chemistry
[SDU.ENVI]Sciences of the Universe [physics]/Continental interfaces, environment
ComputingMilieux_MISCELLANEOUS
0105 earth and related environmental sciences
Aqueous solution
Chemistry
Ligand
Electrochemical Techniques
[CHIM.MATE]Chemical Sciences/Material chemistry
Models, Theoretical
6. Clean water
030104 developmental biology
[CHIM.POLY]Chemical Sciences/Polymers
Stability constants of complexes
visual_art
visual_art.visual_art_medium
Biophysics
Intracellular
Water Pollutants, Chemical
Cadmium
Subjects
Details
- Language :
- English
- ISSN :
- 14639076 and 14639084
- Database :
- OpenAIRE
- Journal :
- Physical Chemistry Chemical Physics, Physical Chemistry Chemical Physics, Royal Society of Chemistry, 2017, 19 (43), pp.29114-29124. ⟨10.1039/c7cp05456d⟩
- Accession number :
- edsair.doi.dedup.....781654dff307ead6428642e305219ed3
- Full Text :
- https://doi.org/10.1039/c7cp05456d⟩