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Differential gene expression in human fibroblasts after alpha-particle emitter211At compared with60Co irradiation
- Source :
- International Journal of Radiation Biology. 89:250-258
- Publication Year :
- 2012
- Publisher :
- Informa UK Limited, 2012.
-
Abstract
- The aim of this study was to identify gene expression profiles distinguishing alpha-particle (211)At and (60)Co irradiation.Gene expression microarray profiling was performed using total RNA from confluent human fibroblasts 5 hours after exposure to (211)At labeled trastuzumab monoclonal antibody (0.25, 0.5, and 1 Gy) and (60)Co (1, 2, and 3 Gy).We report gene expression profiles that distinguish the effect different radiation qualities and absorbed doses have on cellular functions in human fibroblasts. In addition, we identified commonly expressed transcripts between (211)At and (60)Co irradiation. A greater number of transcripts were modulated by (211)At than (60)Co irradiation. In addition, down-regulation was more prevalent than up-regulation following (211)At irradiation. Several biological processes were enriched for both irradiation qualities such as transcription, cell cycle regulation, and cell cycle arrest, whereas mitosis, spindle assembly checkpoint, and apoptotic chromosome condensation were uniquely enriched for alpha particle irradiation.LET-dependent transcriptional modulations were observed in human fibroblasts 5 hours after irradiation exposure. These findings suggest that in comparison with (60)Co, (211)At has the clearest influence on both tumor protein p53-activated and repressed genes, which impose a greater overall burden to the cell following alpha particle irradiation.
- Subjects :
- Time Factors
Cell cycle checkpoint
Transcription, Genetic
Radiological and Ultrasound Technology
medicine.drug_class
Dose-Response Relationship, Radiation
Fibroblasts
Biology
Cell cycle
Alpha Particles
Monoclonal antibody
Molecular biology
Cell Line
Spindle checkpoint
Transcription (biology)
Cell culture
Gene expression
medicine
Humans
Linear Energy Transfer
Radiology, Nuclear Medicine and imaging
Cobalt Radioisotopes
Transcriptome
Astatine
Mitosis
Subjects
Details
- ISSN :
- 13623095 and 09553002
- Volume :
- 89
- Database :
- OpenAIRE
- Journal :
- International Journal of Radiation Biology
- Accession number :
- edsair.doi.dedup.....77ee2c5ef187c74c69118788c1fb636b