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Identification of a Novel Linear B Cell Epitope on the Sao Protein of Streptococcus suis Serotype 2
- Source :
- Frontiers in Immunology, Frontiers in Immunology, Vol 11 (2020)
- Publication Year :
- 2020
- Publisher :
- Frontiers Media SA, 2020.
-
Abstract
- Surface antigen one (Sao) protein is a bacterial surface protein identified in the important zoonotic pathogen Streptococcus suis serotype 2 (S. suis 2) during an extensive search for functional proteins. The Sao protein is anchored to the bacterial cell wall by the LPVTG motif and is widely distributed in many S. suis serotypes. In this paper, we present the immunodominant epitope peptide of the Sao protein that is recognized by BALB/c antibodies against the Sao protein: 355SEKQMPSVVNENAVTPEKQMTNKENDNIET384 (location Sao355−384). To determine the core epitope recognized by antibodies, we prepared truncation peptide libraries. Analyses of the immunoreactivity of truncation peptides with anti-Sao355−384 serum revealed that the most immunoreactive sequence was 355SEKQMPSVVNENAVTPEK372 (location Sao355−372). Moreover, we observed that this core epitope also showed good specificity based on the ratio of reactivity with serum from S. suis–positive patients compared to serum from S. suis–negative patients. Our results point to the potential of using the Sao355−372 peptide in diagnostic assays to determine S. suis infection in humans.
- Subjects :
- lcsh:Immunologic diseases. Allergy
0301 basic medicine
Serotype
Bacterial Zoonoses
Streptococcus suis
Amino Acid Motifs
Immunology
Peptide
Biology
surface antigen one
Epitope
Microbiology
Mice
03 medical and health sciences
0302 clinical medicine
Bacterial Proteins
Antigen
Cell Wall
Peptide Library
Streptococcal Infections
synthetic peptide
medicine
Animals
Humans
Immunology and Allergy
Serologic Tests
B cell
Original Research
chemistry.chemical_classification
Mice, Inbred BALB C
Streptococcus suis serotype 2
Membrane Proteins
B-cell epitope
biology.organism_classification
Antibodies, Bacterial
030104 developmental biology
medicine.anatomical_structure
chemistry
Antigens, Surface
biology.protein
Epitopes, B-Lymphocyte
homology analysis
Female
ELISA
Antibody
lcsh:RC581-607
Protein Binding
030215 immunology
Subjects
Details
- ISSN :
- 16643224
- Volume :
- 11
- Database :
- OpenAIRE
- Journal :
- Frontiers in Immunology
- Accession number :
- edsair.doi.dedup.....77ec685ef311d19553bd08894eb158e7