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Evidence for contamination as the origin for bacteria found in human placenta rather than a microbiota
- Source :
- PLoS ONE, PLoS ONE, 2020, 15 (8), pp.e0237232. ⟨10.1371/journal.pone.0237232⟩, PLoS ONE, Vol 15, Iss 8, p e0237232 (2020)
- Publication Year :
- 2020
- Publisher :
- HAL CCSD, 2020.
-
Abstract
- International audience; Until recently the in utero environment of pregnant women was considered sterile. Recent high-sensitivity molecular techniques and high-throughput sequencing lead to some evidence for a low-biomass microbiome associated with the healthy placenta. Other studies failed to reveal evidence for a consistent presence of bacteria using either culture or molecular based techniques. Comparing conflicting "placental microbiome" studies is complicated by the use of varied and inconsistent protocols. Given this situation, we undertook an evaluation of the in utero environment sterility using several controlled methods, in the same study, to evaluate the presence or absence of bacteria and to explain contradictions present in the literature. Healthy pregnant women (n = 38) were recruited in three maternity wards. Placenta were collected after cesarean section with or without Alexis ® and vaginal delivery births. For this study we sampled fetal membranes, umbilical cord and chorionic villi. Bacterial presence was analyzed using bacterial culture and qPCR on 34 fetal membranes, umbilical cord and chorionic villi samples. Shotgun metagenomics was performed on seven chorionic villi samples. We showed that the isolation of meaningful quantities of viable bacteria or bacterial DNA was possible only outside the placenta (fetal membranes and umbilical cords) highlighting the importance of sampling methods in studying the in utero environment. Bacterial communities described by metagenomics analysis were similar in chorionic villi samples and in negative controls and were dependent on the database chosen for the analysis. We conclude that the placenta does not harbor a specific, consistent and functional microbiota.
- Subjects :
- 0301 basic medicine
Embryology
Placenta
Extraembryonic Membranes
Physiology
Umbilical cord
Umbilical Cord
Database and Informatics Methods
0302 clinical medicine
MESH: Pregnancy
Pregnancy
Medicine and Health Sciences
MESH: Chorionic Villi
DNA extraction
reproductive and urinary physiology
030219 obstetrics & reproductive medicine
Multidisciplinary
medicine.diagnostic_test
Microbiota
Genomics
MESH: Cesarean Section
MESH: Placenta
MESH: Chorionic Villi Sampling
medicine.anatomical_structure
[SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology
Chorionic Villi Sampling
Obstetric Procedures
Medical Microbiology
In utero
embryonic structures
Chorionic villi
Medicine
Female
Chorionic Villi
Sequence Analysis
Research Article
Adult
DNA, Bacterial
Bioinformatics
Science
Sequence Databases
Chorionic villus sampling
Surgical and Invasive Medical Procedures
Microbial Genomics
Biology
Microbiology
Specimen Handling
03 medical and health sciences
Extraction techniques
Genetics
medicine
Humans
MESH: Microbiota
Microbiome
MESH: Specimen Handling
[SDV.MP] Life Sciences [q-bio]/Microbiology and Parasitology
Fetus
MESH: Humans
Bacteria
MESH: Umbilical Cord
Cesarean Section
MESH: Extraembryonic Membranes
Gut Bacteria
Organisms
Biology and Life Sciences
MESH: Adult
Delivery, Obstetric
medicine.disease
MESH: DNA, Bacterial
Research and analysis methods
MESH: Bacteria
Biological Databases
030104 developmental biology
MESH: Delivery, Obstetric
Metagenomics
MESH: Female
Developmental Biology
Subjects
Details
- Language :
- English
- ISSN :
- 19326203
- Database :
- OpenAIRE
- Journal :
- PLoS ONE, PLoS ONE, 2020, 15 (8), pp.e0237232. ⟨10.1371/journal.pone.0237232⟩, PLoS ONE, Vol 15, Iss 8, p e0237232 (2020)
- Accession number :
- edsair.doi.dedup.....77d60a808dc131361fe399310ee504dc