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Topologically correct synthetic reconstruction of pathogen social behavior found during Yersinia growth in deep tissue sites
- Source :
- eLife, eLife, Vol 9 (2020)
- Publication Year :
- 2020
- Publisher :
- eLife Sciences Publications, Ltd, 2020.
-
Abstract
- Within deep tissue sites, extracellular bacterial pathogens often replicate in clusters that are surrounded by immune cells. Disease is modulated by interbacterial interactions as well as bacterial-host cell interactions resulting in microbial growth, phagocytic attack and secretion of host antimicrobial factors. To overcome the limited ability to manipulate these infection sites, we established a system for Yersinia pseudotuberculosis (Yptb) growth in microfluidics-driven microdroplets that regenerates microbial social behavior in tissues. Chemical generation of nitric oxide (NO) in the absence of immune cells was sufficient to reconstruct microbial social behavior, as witnessed by expression of the NO-inactivating protein Hmp on the extreme periphery of microcolonies, mimicking spatial regulation in tissues. Similarly, activated macrophages that expressed inducible NO synthase (iNOS) drove peripheral expression of Hmp, allowing regeneration of social behavior observed in tissues. These results argue that topologically correct microbial tissue growth and associated social behavior can be reconstructed in culture.
- Subjects :
- droplets
QH301-705.5
Science
Cell
microfluidics
Inflammation
Biology
Nitric Oxide
Models, Biological
General Biochemistry, Genetics and Molecular Biology
Nitric oxide
social behavior
chemistry.chemical_compound
Immune system
Immunology and Inflammation
Lab-On-A-Chip Devices
medicine
Extracellular
Yersinia pseudotuberculosis
Secretion
Biology (General)
Pathogen
Microbiology and Infectious Disease
General Immunology and Microbiology
General Neuroscience
Macrophages
General Medicine
biology.organism_classification
Yersinia
Cell biology
Tools and Resources
medicine.anatomical_structure
chemistry
Host-Pathogen Interactions
Medicine
Microbial Interactions
Other
medicine.symptom
Subjects
Details
- Language :
- English
- ISSN :
- 2050084X
- Volume :
- 9
- Database :
- OpenAIRE
- Journal :
- eLife
- Accession number :
- edsair.doi.dedup.....77c3901436877da67489976a81a5d4cf