Back to Search
Start Over
Chlamydophila felis CF0218 Is a Novel TMH Family Protein with Potential as a Diagnostic Antigen for Diagnosis of C. felis Infection
- Source :
- Clinical and Vaccine Immunology. 15:1606-1615
- Publication Year :
- 2008
- Publisher :
- American Society for Microbiology, 2008.
-
Abstract
- Chlamydophila felis is a causative agent of acute and chronic conjunctivitis and pneumonia in cats (feline chlamydiosis). Also, C. felis is a suspected zoonotic agent of such diseases as non- Chlamydia trachomatis conjunctivitis in humans, although this is controversial. At present, there is no serodiagnostic system that specifically detects C. felis infection conveniently. Current systems use antigens such as lipopolysaccharide that cross-react with all chlamydia species. In addition, it is difficult to distinguish between cats that are vaccinated with the commercial vaccine against C. felis and cats that are infected with C. felis . Here, we describe a new candidate diagnostic antigen for diagnosis of C. felis infection, CF0218, that was obtained by screening a genomic expression library of C. felis Fe/C-56 with C. felis -immunized serum. CF0218 was a putative transmembrane head (TMH) family protein with bilobed hydrophobic motifs at its N terminus, and orthologues of CF0218 were not found in the Chlamydophila pneumoniae or Chlamydia trachomatis genomes. The recombinant CF0218 was not recognized by antiserum against C. trachomatis , suggesting that CF0218 is C. felis specific. CF0218 transcription during the course of C. felis infection was confirmed by reverse transcription-PCR. By indirect immunofluorescence analysis, CF0218 was colocalized with the C. felis -formed inclusion bodies in the infected cells. The antibody response against CF0218 was elevated following C. felis infection but not by vaccination in experimentally vaccinated and infected cats. These results suggest that CF0218, a novel TMH family protein of C. felis , possesses potential as a C. felis infection-specific diagnostic antigen.
- Subjects :
- DNA, Bacterial
Microbiology (medical)
Molecular Sequence Data
Clinical Biochemistry
Immunology
Cat Diseases
medicine.disease_cause
Antigen
Chlamydophila felis
Gene Order
medicine
Animals
Clinical Laboratory Immunology
Immunology and Allergy
Amino Acid Sequence
Chlamydophila Infections
Antiserum
Antigens, Bacterial
Chlamydophila
biology
Felis
Membrane Proteins
Pneumonia
Sequence Analysis, DNA
Conjunctivitis
biology.organism_classification
Antibodies, Bacterial
Virology
Genes, Bacterial
Chlamydophila pneumoniae
Cats
biology.protein
Antibody
Chlamydia trachomatis
Sequence Alignment
Subjects
Details
- ISSN :
- 1556679X and 15566811
- Volume :
- 15
- Database :
- OpenAIRE
- Journal :
- Clinical and Vaccine Immunology
- Accession number :
- edsair.doi.dedup.....77bc3060f1a8dd7c11b1e790720eb13b