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CRISPR-SKIP: programmable gene splicing with single base editors
- Source :
- Genome Biology, Genome Biology, Vol 19, Iss 1, Pp 1-11 (2018)
- Publication Year :
- 2018
- Publisher :
- Springer Science and Business Media LLC, 2018.
-
Abstract
- CRISPR gene editing has revolutionized biomedicine and biotechnology by providing a simple means to engineer genes through targeted double-strand breaks in the genomic DNA of living cells. However, given the stochasticity of cellular DNA repair mechanisms and the potential for off-target mutations, technologies capable of introducing targeted changes with increased precision, such as single-base editors, are preferred. We present a versatile method termed CRISPR-SKIP that utilizes cytidine deaminase single-base editors to program exon skipping by mutating target DNA bases within splice acceptor sites. Given its simplicity and precision, CRISPR-SKIP will be broadly applicable in gene therapy and synthetic biology. Electronic supplementary material The online version of this article (10.1186/s13059-018-1482-5) contains supplementary material, which is available to authorized users.
- Subjects :
- 0301 basic medicine
lcsh:QH426-470
Base pair
Method
RELA
Computational biology
Biology
Cell Line
Base editing
03 medical and health sciences
Synthetic biology
0302 clinical medicine
Genome editing
Consensus Sequence
Humans
CRISPR
Clustered Regularly Interspaced Short Palindromic Repeats
Gene isoform
lcsh:QH301-705.5
Base Pairing
Gene
Gene Editing
Genome
Base Sequence
Alternative splicing
High-Throughput Nucleotide Sequencing
Exons
PIK3CA
Cytidine deaminase
BRCA2
Exon skipping
lcsh:Genetics
030104 developmental biology
lcsh:Biology (General)
RNA Splice Sites
CRISPR-Cas9
030217 neurology & neurosurgery
Subjects
Details
- ISSN :
- 1474760X
- Volume :
- 19
- Database :
- OpenAIRE
- Journal :
- Genome Biology
- Accession number :
- edsair.doi.dedup.....77a90eab7f30ae715d9d1fe40adfd197