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Changes in the Proanthocyanidin Composition and Related Gene Expression in Bilberry (Vaccinium myrtillus L.) Tissues

Authors :
Kaisu Riihinen
Laura Jaakola
Katja Karppinen
Jussi Suvanto
Juha-Pekka Salminen
Source :
Journal of Agricultural and Food Chemistry, Suvanto, J, Karppinen, K, Riihinen, K, Jaakola, L & Salminen, J P 2020, ' Changes in the Proanthocyanidin Composition and Related Gene Expression in Bilberry (Vaccinium myrtillus L.) Tissues ', Journal of Agricultural and Food Chemistry, vol. 68, no. 28, pp. 7378-7386 . https://doi.org/10.1021/acs.jafc.0c02158
Publication Year :
2020
Publisher :
ACS Publications, 2020.

Abstract

Berries of genus Vaccinium are rich in flavonoids and proanthocyanidins (PAs). We studied the PA composition and biosynthesis in bilberry (Vaccinium myrtillus L.) tissues and during fruit development. Soluble PAs, analyzed by UHPLC–MS/MS, were most abundant in stem and rhizome with the mean PA polymerization level varying between 4 and 6 in all tissues. Both A- and B-type PAs were present in all tissues. Procyanidin subunits were more common than prodelphinidin subunits in PAs. During fruit ripening, the amount of procyanidin subunits decreased while prodelphinidin subunits and F3′5′H expression increased, indicating a shift in biosynthesis toward the delphinidin branch of the flavonoid pathway. Epicatechin was the most abundant flavan-3-ol in all tissues. Expression of ANR and three isolated LAR genes, analyzed by qRT-PCR, showed connection to accumulation of PAs and flavan-3-ols biosynthesized from different flavonoid branches. Insoluble PAs accumulated during berry development, suggesting that PAs are not recycled after biosynthesis.

Details

Language :
English
Database :
OpenAIRE
Journal :
Journal of Agricultural and Food Chemistry, Suvanto, J, Karppinen, K, Riihinen, K, Jaakola, L & Salminen, J P 2020, ' Changes in the Proanthocyanidin Composition and Related Gene Expression in Bilberry (Vaccinium myrtillus L.) Tissues ', Journal of Agricultural and Food Chemistry, vol. 68, no. 28, pp. 7378-7386 . https://doi.org/10.1021/acs.jafc.0c02158
Accession number :
edsair.doi.dedup.....77a1b57ede74ca4d3f3b46775e6d108c
Full Text :
https://doi.org/10.1021/acs.jafc.0c02158