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Changes in the Proanthocyanidin Composition and Related Gene Expression in Bilberry (Vaccinium myrtillus L.) Tissues
- Source :
- Journal of Agricultural and Food Chemistry, Suvanto, J, Karppinen, K, Riihinen, K, Jaakola, L & Salminen, J P 2020, ' Changes in the Proanthocyanidin Composition and Related Gene Expression in Bilberry (Vaccinium myrtillus L.) Tissues ', Journal of Agricultural and Food Chemistry, vol. 68, no. 28, pp. 7378-7386 . https://doi.org/10.1021/acs.jafc.0c02158
- Publication Year :
- 2020
- Publisher :
- ACS Publications, 2020.
-
Abstract
- Berries of genus Vaccinium are rich in flavonoids and proanthocyanidins (PAs). We studied the PA composition and biosynthesis in bilberry (Vaccinium myrtillus L.) tissues and during fruit development. Soluble PAs, analyzed by UHPLC–MS/MS, were most abundant in stem and rhizome with the mean PA polymerization level varying between 4 and 6 in all tissues. Both A- and B-type PAs were present in all tissues. Procyanidin subunits were more common than prodelphinidin subunits in PAs. During fruit ripening, the amount of procyanidin subunits decreased while prodelphinidin subunits and F3′5′H expression increased, indicating a shift in biosynthesis toward the delphinidin branch of the flavonoid pathway. Epicatechin was the most abundant flavan-3-ol in all tissues. Expression of ANR and three isolated LAR genes, analyzed by qRT-PCR, showed connection to accumulation of PAs and flavan-3-ols biosynthesized from different flavonoid branches. Insoluble PAs accumulated during berry development, suggesting that PAs are not recycled after biosynthesis.
- Subjects :
- 0106 biological sciences
Bilberry
Flavonoid
Vaccinium myrtillus
01 natural sciences
Article
chemistry.chemical_compound
SDG 3 - Good Health and Well-being
Gene Expression Regulation, Plant
VDP::Mathematics and natural science: 400::Zoology and botany: 480
flavonoid biosynthesis
Plant Proteins
chemistry.chemical_classification
biology
flavan-3-ols
Chemistry
010401 analytical chemistry
food and beverages
General Chemistry
biology.organism_classification
0104 chemical sciences
Flavonoid biosynthesis
Biochemistry
Proanthocyanidin
Fruit
gene expression
fruit development
Delphinidin
General Agricultural and Biological Sciences
proanthocyanidins
Prodelphinidin
010606 plant biology & botany
Vaccinium
VDP::Matematikk og Naturvitenskap: 400::Zoologiske og botaniske fag: 480
Subjects
Details
- Language :
- English
- Database :
- OpenAIRE
- Journal :
- Journal of Agricultural and Food Chemistry, Suvanto, J, Karppinen, K, Riihinen, K, Jaakola, L & Salminen, J P 2020, ' Changes in the Proanthocyanidin Composition and Related Gene Expression in Bilberry (Vaccinium myrtillus L.) Tissues ', Journal of Agricultural and Food Chemistry, vol. 68, no. 28, pp. 7378-7386 . https://doi.org/10.1021/acs.jafc.0c02158
- Accession number :
- edsair.doi.dedup.....77a1b57ede74ca4d3f3b46775e6d108c
- Full Text :
- https://doi.org/10.1021/acs.jafc.0c02158