Back to Search
Start Over
Morphological and genetic identification of fungal genus/species in formalinâfixed, paraffinâembedded specimens obtained from patients with histologically proven fungal infection
- Source :
- Mycoses. 64:851-859
- Publication Year :
- 2021
- Publisher :
- Wiley, 2021.
-
Abstract
- Background Although fungi are found relatively easily by microscopic examination of pathological samples, identification of fungal genus and species in pathological samples is not easy because the morphological features of fungi are similar among genera and species. Objectives A multiple real-time PCR was developed for identification of fungal genus/species, and morphological characterizations of fungi were analyzed in pathological samples. Patients/methods Seventy-five formalin-fixed, paraffin-embedded samples morphologically proven to contain any fungus were examined. A multiple real-time PCR system was developed to identify 25 fungal genus/species in pathological samples. Morphology of fungus in the specimens was re-reviewed retrospectively based on the results of real-time PCR. Results Real-time PCR identified fungal genus/species in 56 of 75 (74.6%) specimens with histologically proven fungal infection. In 53 specimens of filamentous fungi, Aspergillus spp. (22 specimens), Cladosporium (8), Scedosporium apiospermum (4), Malassezia sympodialis (1) and Candida albicans (1) were identified. Pseudohyphae of Candida were confused with filamentous fungus in a case. Morphological observation suggested differences in the presence of septated or non-septated hyphae, the filament size, and the branch angle among genus/species of filamentous fungi; however, genus/species was not able to be determined by their morphological features. In 22 specimens of yeasts, real-time PCR allowed for the identification of Candida albicans (12 specimens), Candida glabrata (2), Cladosporium (2), Scedosporium apiospermum (2), Pichia kudriavzevii (1), and Aspergillus sydowii (1). Conclusions These data suggest that it is difficult to identify fungal genus/species by morphological features alone. Real-time PCR is useful to identify fungal genus/species in pathological samples.
- Subjects :
- 0301 basic medicine
Hypha
030106 microbiology
Dermatology
Microbiology
030207 dermatology & venereal diseases
03 medical and health sciences
0302 clinical medicine
Genus
Formaldehyde
Humans
DNA, Fungal
Candida albicans
Retrospective Studies
Aspergillus
Paraffin Embedding
biology
Candida glabrata
Fungi
Scedosporium apiospermum
General Medicine
biology.organism_classification
Infectious Diseases
Molecular Diagnostic Techniques
Mycoses
Malassezia sympodialis
Female
Multiplex Polymerase Chain Reaction
Cladosporium
Subjects
Details
- ISSN :
- 14390507 and 09337407
- Volume :
- 64
- Database :
- OpenAIRE
- Journal :
- Mycoses
- Accession number :
- edsair.doi.dedup.....77862f22c548d38b3cfe9fc2b413a67c