Back to Search
Start Over
A Transport Form of Collagen from Embryonic Tendon: Electron Microscopic Demonstration of an NH2-Terminal Extension and Evidence Suggesting the Presence of Cystine in the Molecule
- Publication Year :
- 1972
-
Abstract
- When cells were isolated from chickembryo tendons and incubated in vitro for 2-6 hr, essentially all the newly-synthesized collagen was recovered from the incubation medium as a transport form larger than tropocollagen. Experiments in which cells were incubated with [ 14 C]cystine suggested that the transport form contained cystine and that it was, in part, stabilized by disulfide bonds. Electron microscopy of segment-long-spacing aggregates prepared from the transport form of collagen showed that the native molecule differed from tropocollagen in that it had an extension of about 13 nm (130 Å) at the NH 2 -terminal end.
- Subjects :
- Tropocollagen
Proline
Protein Conformation
Size-exclusion chromatography
Detergents
Cystine
Chick Embryo
law.invention
Tendons
chemistry.chemical_compound
Protein structure
law
medicine
Animals
Trypsin
Carbon Isotopes
Multidisciplinary
Sulfates
Biological Transport
In vitro
Pepsin A
Microscopy, Electron
Microbial Collagenase
chemistry
Biochemistry
Ammonium Sulfate
Chromatography, Gel
Biological Sciences: Biochemistry
Collagen
Electron microscope
Anura
Carrier Proteins
medicine.drug
Subjects
Details
- Language :
- English
- Database :
- OpenAIRE
- Accession number :
- edsair.doi.dedup.....77563f97469b574d612c4aaec6500805