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The clinical role of epithelial-mesenchymal transition and stem cell markers in advanced-stage ovarian serous carcinoma effusions
- Source :
- Human Pathology. 46:1-8
- Publication Year :
- 2015
- Publisher :
- Elsevier BV, 2015.
-
Abstract
- We recently identified gene signatures that allow classification of ovarian carcinoma into 5 distinct clinically relevant groups. In the present study, we investigated the clinical role of 10 protein products of the discriminating genes, with focus on epithelial-mesenchymal transition and stem cell markers. Expression of E-cadherin, N-cadherin, P-cadherin, Zeb1, HMGA2, Rab25, CD24, NCAM (CD56), Sox11, and vimentin was assessed in 100 advanced-stage (International Federation of Gynecology and Obstetrics stages III-IV) serous ovarian carcinoma effusions using immunohistochemistry. Results were analyzed for association with clinicopathological parameters, including chemotherapy response, and survival. All 10 proteins were frequently expressed in carcinoma cells. HMGA2 expression was related to older age (P = .015). HMGA2 and NCAM expression was related to stage III disease (P = .011 and P = .023, respectively), and NCAM was overexpressed in peritoneal compared with pleural effusions (P = .001). Vimentin and Zeb1 expression was significantly related to poor chemotherapy response at diagnosis (P = .005 and P = .017, respectively). The associations between NCAM and peritoneal localization and of vimentin and poor chemoresponse were retained after Bonferroni correction. NCAM expression was associated with a trend for shorter overall survival in univariate survival analysis (P = .187), but emerged as an independent prognosticator in Cox multivariate analysis (P = .042). This study identifies vimentin and Zeb1 as markers of poor chemoresponse in metastatic serous ovarian carcinoma effusions and suggests NCAM as potential prognostic marker in metastatic disease. The generally limited prognostic role of the studied markers emphasizes the difficulty in applying data obtained in studies of primary ovarian carcinomas to analyses of ovarian carcinoma effusions, reflecting the unique biology of the latter.
- Subjects :
- Adult
Pathology
medicine.medical_specialty
Epithelial-Mesenchymal Transition
Time Factors
Biopsy
Antineoplastic Agents
Vimentin
Stem cell marker
Pathology and Forensic Medicine
HMGA2
Predictive Value of Tests
Risk Factors
Ovarian carcinoma
Biomarkers, Tumor
medicine
Carcinoma
Humans
Epithelial–mesenchymal transition
Neural Cell Adhesion Molecules
Aged
Neoplasm Staging
Proportional Hazards Models
Aged, 80 and over
Homeodomain Proteins
Ovarian Neoplasms
Chi-Square Distribution
biology
CD24
business.industry
Age Factors
Zinc Finger E-box-Binding Homeobox 1
Middle Aged
medicine.disease
Immunohistochemistry
P-Selectin
Serous fluid
Treatment Outcome
Drug Resistance, Neoplasm
Multivariate Analysis
Neoplastic Stem Cells
biology.protein
Female
Neoplasm Grading
Neoplasms, Cystic, Mucinous, and Serous
business
Transcription Factors
Subjects
Details
- ISSN :
- 00468177
- Volume :
- 46
- Database :
- OpenAIRE
- Journal :
- Human Pathology
- Accession number :
- edsair.doi.dedup.....77546fe8757c6e69edaf749d59a0f35a