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Differential effects of low and high doses of lipoteichoic acid on lipopolysaccharide-induced interleukin-6 production
- Source :
- Inflammation Research. 63:419-428
- Publication Year :
- 2014
- Publisher :
- Springer Science and Business Media LLC, 2014.
-
Abstract
- Interleukin-6 (IL-6), which is increased in patients who are suffering from septic shock, is an important mediator of the inflammatory response. Here, we examined the priming effect of lipoteichoic acid (LTA) and lipopolysaccharide (LPS) on IL-6 production in a monocyte-like cell line. THP-1 cells were primed by treatingwith a low or high dose of LTA isolated from Staphylococcus aureus (aLTA) and then re-treated with LPS. IL-6 production, receptor expression, and the variation of signaling molecules were examined by ELISA, reverse transcriptase polymerase chain reaction, and western blotting, respectively. LPS-mediated IL-6 production was dramatically increased in THP-1 cells pretreated with a low dose aLTA, while it was significantly decreased when a high dose of aLTA was given along with LPS. LPS-induced IL-6 production in low dose aLTA priming cells mediated by NF-κB and MAPKs pathways, and Akt functioned as a negative regulator of IL-6 production. Together, the results of this study suggest that different doses of bacterial cell surface components can mediate a diverse range of responses with respect to inflammatory cytokine production.
- Subjects :
- Lipopolysaccharides
Staphylococcus aureus
Lipopolysaccharide
medicine.medical_treatment
Receptor expression
Immunology
Microbiology
chemistry.chemical_compound
Cell Line, Tumor
medicine
Humans
Interleukin 6
Protein kinase B
Pharmacology
biology
Interleukin-6
Chemistry
NF-kappa B
Molecular biology
Teichoic Acids
Blot
Cytokine
Cell culture
biology.protein
lipids (amino acids, peptides, and proteins)
Lipoteichoic acid
Mitogen-Activated Protein Kinases
Proto-Oncogene Proteins c-akt
Lactobacillus plantarum
Subjects
Details
- ISSN :
- 1420908X and 10233830
- Volume :
- 63
- Database :
- OpenAIRE
- Journal :
- Inflammation Research
- Accession number :
- edsair.doi.dedup.....7732d1c0b82733a4aa3481774ad98786