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Altered Gene Regulatory Networks are Associated with the Transition from C3 to Crassulacean Acid Metabolism in Erycina (Oncidiinae: Orchidaceae)
- Source :
- Frontiers in Plant Science, Vol 9 (2019)
- Publication Year :
- 2018
- Publisher :
- Cold Spring Harbor Laboratory, 2018.
-
Abstract
- Crassulacean acid metabolism (CAM) photosynthesis is a modification of the core C3 photosynthetic pathway that improves the ability of plants to assimilate carbon in water-limited environments. CAM plants fix CO2 mostly at night, when transpiration rates are low. All of the CAM pathway genes exist in ancestral C3 species, but the timing and magnitude of expression are greatly altered between C3 and CAM species. Understanding these regulatory changes is key to elucidating the mechanism by which CAM evolved from C3. Here we use two closely related species in the Orchidaceae, Erycina pusilla (CAM) and Erycina crista-galli (C3), to conduct comparative transcriptomic analyses across multiple time points. Clustering of genes with expression variation across the diel cycle revealed some canonical CAM pathway genes similarly expressed in both species, regardless of photosynthetic pathway. However, gene network construction indicated that 149 gene families had significant differences in network connectivity and were further explored for these functional enrichments. Genes involved in light sensing and ABA signaling were some of the most differently connected genes between the C3 and CAM Erycina species, in agreement with the contrasting diel patterns of stomatal conductance in C3 and CAM plants. Our results suggest changes to transcriptional cascades are important for the transition from C3 to CAM photosynthesis in Erycina.
- Subjects :
- 0106 biological sciences
0301 basic medicine
Stomatal conductance
Gene regulatory network
RNA-Seq
Plant Science
lcsh:Plant culture
Biology
Photosynthesis
01 natural sciences
Transcriptome
transcriptomics
03 medical and health sciences
Gene family
time-course
lcsh:SB1-1110
Transcriptomics
Gene
030304 developmental biology
Transpiration
Genetics
0303 health sciences
photosynthesis
Science::Biological sciences [DRNTU]
030104 developmental biology
gene network
Crassulacean acid metabolism
RNA-seq
010606 plant biology & botany
Subjects
Details
- Language :
- English
- Database :
- OpenAIRE
- Journal :
- Frontiers in Plant Science, Vol 9 (2019)
- Accession number :
- edsair.doi.dedup.....77271a39ae4f8937f1112fd13e22431b
- Full Text :
- https://doi.org/10.1101/431460