Identification of Tengfu Jiangya Tablet Target Biomarkers with Quantitative Proteomic Technique

Tengfu Jiangya Tablet (TJT) is a well accepted antihypertension drug in China and its major active components wereUncaria total alkaloidsandSemen Raphani soluble alkaloid. To further explore treatment effects mechanism of TJT on essential hypertension, a serum proteomic study was performed. Potential biomarkers were quantified in serum of hypertension individuals before and after taking TJT with isobaric tags for relative and absolute quantitation (iTRAQ) coupled two-dimensional liquid chromatography followed electrospray ionization-tandem mass spectrometry (2D LC-MS/MS) proteomics technique. Among 391 identified proteins with high confidence, 70 proteins were differentially expressed (fold variation criteria, >1.2 or