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Perfusion and cyclic compression of mesenchymal cell-loaded and clinically applicable osteochondral grafts
- Source :
- Knee Surgery, Sports Traumatology, Arthroscopy. 17:1384-1392
- Publication Year :
- 2009
- Publisher :
- Springer Science and Business Media LLC, 2009.
-
Abstract
- Osteochondral lesions are often seen in orthopedics, but the available treatment strategies are limited in success. Regenerative medicine provides novel concepts for curing them. The purpose of this study was to test the effects of perfusion and cyclic compression on cell differentiation and mechanical properties using a custom-made biomechanoreactor in a recently established system of human bone marrow stromal cells (hBMSC) cultured in a 3-D collagen I-bone hybrid matrix out of commercially available and separately in human-certified products. Seeded hBMSC were viable for 88 +/- 8.9% during the entire experimental period in the constructs. GAG and DNA levels did not change. Perfusion induced collagen II and cyclic compression increased collagen X expression. Matrix stiffness was significantly increased after 28 days of cyclic compression. Cyclic compression of cell-loaded hybrid constructs enhanced chondrocyte differentiation and matrix stiffness. This system is a promising tool with a view to a later clinical application.
- Subjects :
- Adult
Male
medicine.medical_specialty
Stromal cell
Cellular differentiation
Bone Marrow Cells
Regenerative medicine
Chondrocyte
Bioreactors
Chondrocytes
Tissue engineering
Humans
Medicine
Orthopedics and Sports Medicine
Cells, Cultured
Bone Transplantation
Tissue Engineering
business.industry
Cartilage
Mesenchymal stem cell
Biomechanical Phenomena
Surgery
Perfusion
medicine.anatomical_structure
Female
Stromal Cells
business
Biomedical engineering
Subjects
Details
- ISSN :
- 14337347 and 09422056
- Volume :
- 17
- Database :
- OpenAIRE
- Journal :
- Knee Surgery, Sports Traumatology, Arthroscopy
- Accession number :
- edsair.doi.dedup.....76d9ef148a886b89aa18938f2c56c35f