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Competitive Interactions of Ionic Surfactants with Salbutamol and Bovine Serum Albumin: A Molecular Spectroscopy Study with Implications for Salbutamol in Food Analysis
- Source :
- Journal of Agricultural and Food Chemistry. 61:7730-7738
- Publication Year :
- 2013
- Publisher :
- American Chemical Society (ACS), 2013.
-
Abstract
- The effect of ionic surfactants, sodium dodecyl sulfate (SDS) and N-cetyl-N,N,N-trimethylammonium bromide (CTAB), on the interaction between β-agonist salbutamol (SAL) and bovine serum albumin (BSA) was investigated with the use of fluorescence spectroscopy (FLS) and chemometrics methods [multivariate curve resolution-alternating least-squares (MCR-ALS) and parallel factor analysis algorithm (PARAFAC)]. It was found that the binding constant of SAL to BSA in the presence of CTAB was much larger than that without this ligand. The ligand/BSA stoichiometry was 4:1, that is, (CTAB)4-BSA, and was 2:1 with the ligand, that is, (SAL)2-BSA. These results were obtained from the concentration profiles extracted by MCR-ALS for all three reactants. Quantitative information on the complex CTAB-BSA-SAL species was obtained with the resolution of the excitation-emission fluorescence three-way data matrices by PARAFAC. This research has implications for the analysis of SAL in food and might be performed in laboratories associated with organizations such as the U.S. Food and Drug Administration (FDA) and the International Olympic Committee (IOC).
- Subjects :
- Serum albumin
Food Contamination
Fluorescence spectroscopy
Chemometrics
Surface-Active Agents
chemistry.chemical_compound
Bromide
parasitic diseases
Animals
Albuterol
Bovine serum albumin
Sodium dodecyl sulfate
Adrenergic beta-2 Receptor Agonists
Chromatography
biology
Chemistry
Serum Albumin, Bovine
General Chemistry
Ligand (biochemistry)
Binding constant
Kinetics
Spectrometry, Fluorescence
biology.protein
Cattle
General Agricultural and Biological Sciences
Protein Binding
Subjects
Details
- ISSN :
- 15205118 and 00218561
- Volume :
- 61
- Database :
- OpenAIRE
- Journal :
- Journal of Agricultural and Food Chemistry
- Accession number :
- edsair.doi.dedup.....76d235488c6607b4a1b224d072687452