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Additional file 1: Figure S1. of MIR-708 promotes phagocytosis to eradicate T-ALL cells by targeting CD47

Authors :
Huang, Wei
Wang, Wen-Tao
Fang, Ke
Chen, Zhen-Hua
Yu-Meng Sun
Han, Cai
Lin-Yu Sun
Luo, Xue-Qun
Chen, Yue-Qin
Publication Year :
2018
Publisher :
figshare, 2018.

Abstract

(A-B). Jurkat cells were electroporated with mimics-NC and mimics-miR-708, The levels of miR-708 was assessed by qRT − PCR and normalized to U6.Cell lysates were prepared for western blotting with the antibody against CD47, and the expression of GAPDH served as a loading control. Figure S2. qRT-PCR analysis of the expressoion of miR-708 and CD47 in B-ALL. U6 and GAPDH were used as endogenous control. Figure S3. Apoptosis assay of CCRF-CEM and Jurkat upon transfection of miR-708 mimics or mimics-NC, respectively. Figure S4. Following the subcutaneous inoculation of CCRF-CEM-LV-NC and CCRF-CEM-LV-miR-708, the levels of miR-708 and CD47 were assessed by qRT − PCR and western blot, respectively.(A-B). Overexpressed miR-708 reduced tumor weight. Error bars reflect ±SEM (five mice, *, p

Details

Database :
OpenAIRE
Accession number :
edsair.doi.dedup.....76d0697cf860ef1bd16a35c0a1c8b560
Full Text :
https://doi.org/10.6084/m9.figshare.5821770