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Cytokine Stimulation Promotes Increased Glucose Uptake Via Translocation at the Plasma Membrane of GLUT1 in HEK293 Cells

Authors :
Paola Murgas
Ilona I. Concha
Constanza Angulo
Clara Jara
Maite A. Castro
Angara Zambrano
Evelyn Jara
Source :
JOURNAL OF CELLULAR BIOCHEMISTRY, Artículos CONICYT, CONICYT Chile, instacron:CONICYT
Publication Year :
2010
Publisher :
Wiley, 2010.

Abstract

Interleukin-3 (IL-3) and granulocyte/macrophage colony-stimulating factor (GM-CSF) are two of the best-characterized cell survival factors in hematopoietic cells; these factors induce an increase in Akt activity in multiple cell lines, a process thought to be involved in cellular survival. It is known that growth factors require sustained glucose metabolism to promote cell survival. It has been determined that IL-3 and GM-CSF signal for increased glucose uptake in hematopoietic cells. Interestingly, receptors for IL-3 and GM-CSF are present in several non-hematopoietic cell types but their roles in these cells have been poorly described. In this study, we demonstrated the expression of IL-3 and GM-CSF receptors in HEK293 cells and analyzed their effect on glucose uptake. In these cells, both IL-3 and GM-CSF, increased glucose uptake. The results indicated that this increase involves the subcellular redistribution of GLUT1, affecting glucose transporter levels at the cell surface in HEK293 cells. Also the data directly demonstrates that the PI 3-kinase/Akt pathway is an important mediator of this process. Altogether these results show a role for non-insulin growth factors in the regulation of GLUT1 trafficking that has not yet been directly determined in non-hematopoietic cells.

Details

ISSN :
07302312
Volume :
110
Database :
OpenAIRE
Journal :
Journal of Cellular Biochemistry
Accession number :
edsair.doi.dedup.....76ad91431f24bc0390f31f31ac5c8053
Full Text :
https://doi.org/10.1002/jcb.22711