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Jasmonic Acid-Dependent MYC Transcription Factors Bind to a Tandem G-Box Motif in the YUCCA8 and YUCCA9 Promoters to Regulate Biotic Stress Responses
- Source :
- International Journal of Molecular Sciences, Vol 22, Iss 9768, p 9768 (2021), International Journal of Molecular Sciences, Volume 22, Issue 18
- Publication Year :
- 2021
- Publisher :
- MDPI AG, 2021.
-
Abstract
- 21 Pàg. Centro de Biotecnología y Genómica de Plantas<br />The indole-3-pyruvic acid pathway is the main route for auxin biosynthesis in higher plants. Tryptophan aminotransferases (TAA1/TAR) and members of the YUCCA family of flavin-containing monooxygenases catalyze the conversion of l-tryptophan via indole-3-pyruvic acid to indole-3-acetic acid (IAA). It has been described that jasmonic acid (JA) locally produced in response to mechanical wounding triggers the de novo formation of IAA through the induction of two YUCCA genes, YUC8 and YUC9. Here, we report the direct involvement of a small number of basic helix-loop-helix transcription factors of the MYC family in this process. We show that the JA-mediated regulation of the expression of the YUC8 and YUC9 genes depends on the abundance of MYC2, MYC3, and MYC4. In support of this observation, seedlings of myc knockout mutants displayed a strongly reduced response to JA-mediated IAA formation. Furthermore, transactivation assays provided experimental evidence for the binding of MYC transcription factors to a particular tandem G-box motif abundant in the promoter regions of YUC8 and YUC9, but not in the promoters of the other YUCCA isogenes. Moreover, we demonstrate that plants that constitutively overexpress YUC8 and YUC9 show less damage after spider mite infestation, thereby underlining the role of auxin in plant responses to biotic stress signals.<br />This research was funded by the Spanish Ministry of Economy, Industry and Competitiveness (MINECO), grant number BFU2017-82826-R
- Subjects :
- Arabidopsis thaliana
QH301-705.5
Catalysis
Inorganic Chemistry
chemistry.chemical_compound
Transactivation
wound response
Biotic stress
Transcriptional regulation
anatomy_morphology
transcriptional regulation
Biology (General)
Physical and Theoretical Chemistry
QD1-999
Molecular Biology
Transcription factor
Spectroscopy
biology
Tandem
Chemistry
Jasmonic acid
jasmonic acid
Organic Chemistry
Biochemistry and Molecular Biology
Botany
food and beverages
Promoter
General Medicine
Monooxygenase
biology.organism_classification
plant hormone crosstalk
Computer Science Applications
Cell biology
indole-3-acetic acid
Indole-3-acetic acid
Growth-defense trade-off
Subjects
Details
- ISSN :
- 14220067
- Volume :
- 22
- Database :
- OpenAIRE
- Journal :
- International Journal of Molecular Sciences
- Accession number :
- edsair.doi.dedup.....76954258e62c7bc5c64198eaeae1e035