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Light-sheet microscopy with isotropic, sub-micron resolution and solvent-independent large-scale imaging
- Publication Year :
- 2019
- Publisher :
- Cold Spring Harbor Laboratory, 2019.
-
Abstract
- We present cleared tissue Axially Swept Light-Sheet Microscopy (ctASLM), which achieves sub-micron isotropic resolution, high optical sectioning capability, and large field of view imaging (870×870 μm2) over a broad range of immersion media. ctASLM can image live, expanded, and both aqueous and organic chemically cleared tissue preparations and provides 2- to 5-fold better axial resolution than confocal or other reported cleared tissue light-sheet microscopes. We image millimeter-sized tissues with sub-micron 3D resolution, which enabled us to perform automated detection of cells and subcellular features such as dendritic spines.
- Subjects :
- 0303 health sciences
Microscope
Materials science
Optical sectioning
business.industry
Confocal
Isotropy
Resolution (electron density)
3. Good health
law.invention
03 medical and health sciences
0302 clinical medicine
Optics
law
Light sheet fluorescence microscopy
Microscopy
business
Axial symmetry
030217 neurology & neurosurgery
030304 developmental biology
Subjects
Details
- Language :
- English
- Database :
- OpenAIRE
- Accession number :
- edsair.doi.dedup.....7678ed274f23d382b0dc6757762772e8
- Full Text :
- https://doi.org/10.1101/605493