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A method for the determination of the residual chloroform in defatted cancellous bone transplants
- Source :
- Cell and Tissue Banking. 6:71-75
- Publication Year :
- 2005
- Publisher :
- Springer Science and Business Media LLC, 2005.
-
Abstract
- The removal of fat from cancellous bone tissue promotes the clinical healing of the transplant and improves the penetration of chemical sterilisation media into the tissue. Treatment using chloroform/methanol (2:1, 2 h) is frequently used as a defatting procedure. Eight rinses with methanol followed by two rinses with aqua ad iniectabilia (20 min each, with ultrasonic effect) ensure depletion in the level of chloroform from defatted cancellous bone to a concentration below 25 ppm (limit value). For the necessary routine quality checks on the production process, a gas chromatography method has been developed that determines the level of chloroform in cancellous bone, for which the detection limit is 0.003 ppm.
- Subjects :
- medicine.medical_specialty
Chromatography, Gas
Biomedical Engineering
Bone and Bones
Defatting
Biomaterials
chemistry.chemical_compound
medicine
Humans
Detection limit
Transplantation
Bone Transplantation
Chloroform
Chromatography
Chemistry
Methanol
Cell Biology
Penetration (firestop)
Lipids
Surgery
medicine.anatomical_structure
Gas chromatography
Cancellous bone
Subjects
Details
- ISSN :
- 15736814 and 13899333
- Volume :
- 6
- Database :
- OpenAIRE
- Journal :
- Cell and Tissue Banking
- Accession number :
- edsair.doi.dedup.....76574d8b858c132c4fb8359a15955812