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Hydrophilic Shell Matrix Proteins of Nautilus pompilius and the Identification of a Core Set of Conchiferan Domains
- Source :
- Genes; Volume 12; Issue 12; Pages: 1925, Genes, Vol 12, Iss 1925, p 1925 (2021), Genes
- Publication Year :
- 2021
- Publisher :
- MDPI AG, 2021.
-
Abstract
- Despite being a member of the shelled mollusks (Conchiferans), most members of extant cephalopods have lost their external biomineralized shells, except for the basally diverging Nautilids. Here, we report the result of our study to identify major Shell Matrix Proteins and their domains in the Nautilid Nautilus pompilius, in order to gain a general insight into the evolution of Conchiferan Shell Matrix Proteins. In order to do so, we performed a multiomics study on the shell of N. pompilius, by conducting transcriptomics of its mantle tissue and proteomics of its shell matrix. Analyses of obtained data identified 61 distinct shell-specific sequences. Of the successfully annotated 27 sequences, protein domains were predicted in 19. Comparative analysis of Nautilus sequences with four Conchiferans for which Shell Matrix Protein data were available (the pacific oyster, the pearl oyster, the limpet and the Euhadra snail) revealed that three proteins and six protein domains were conserved in all Conchiferans. Interestingly, when the terrestrial Euhadra snail was excluded, another five proteins and six protein domains were found to be shared among the four marine Conchiferans. Phylogenetic analyses indicated that most of these proteins and domains were probably present in the ancestral Conchiferan, but employed in shell formation later and independently in most clades. Even though further studies utilizing deeper sequencing techniques to obtain genome and full-length sequences, and functional analyses, must be carried out in the future, our results here provide important pieces of information for the elucidation of the evolution of Conchiferan shells at the molecular level.
- Subjects :
- Protein domain
Shell (structure)
QH426-470
Biology
Proteomics
Article
Evolution, Molecular
proteomics
Protein Domains
Animal Shells
Genetics
Animals
shell evolution
Nautilus
Mantle (mollusc)
Conserved Sequence
Phylogeny
Genetics (clinical)
Phylogenetic tree
Gene Expression Profiling
Limpet
Proteins
Pacific oyster
biomineralization
biology.organism_classification
multiomics
Mollusca
Cephalopoda
Evolutionary biology
Subjects
Details
- ISSN :
- 20734425
- Volume :
- 12
- Database :
- OpenAIRE
- Journal :
- Genes
- Accession number :
- edsair.doi.dedup.....7655ac11c95409ecbf899ffb334d2ffc
- Full Text :
- https://doi.org/10.3390/genes12121925