Studies of folic acid compounds in nature. IV. Folic acid compounds in soybeans and cow milk

Folate compounds in soybean and cow's milk were identified by Sephadex chromatography and differential microbiological assay. Soybean contained mainly monoglutamates (ca. 52%), some diglutamates (ca. 16%), and polyglutamates (pentaglutamates representing the major portion). 5-CHO-H4PteGlun(1–6) constituted 65–70% of total folate activity. Cow's milk contained monoglutamates (60%) and polyglutamates (ranging from di- to hepta-conjugates). In contrast to soybean, 90–95% of milk folate was in the 5-CH3-H4PteGlun(1–7) form.