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Relative quantitation of phosphatidylcholines with interfered masses of protonated and sodiated molecules by tandem and Fourier-transform ion cyclotron resonance mass spectrometry
- Source :
- European Journal of Mass Spectrometry. 25:259-264
- Publication Year :
- 2019
- Publisher :
- SAGE Publications, 2019.
-
Abstract
- The present work deals with deconvolution of interfered peaks of protonated and sodiated phosphatidylcholines to solve the problem of relative quantitation of these compounds. The method was developed based on the fact that adducts of phosphatidylcholines with proton and sodium ion give unique characteristic peaks in tandem mass spectrometry. Ultra-high-resolution Fourier-transform ion cyclotron resonance mass spectrometry was used to resolve interfered peaks and thus validate the results of tandem mass spectrometry-based deconvolution. Applicability of the method was tested with synthesized phosphatidylcholines and applied for tissue-spray analysis of the clinical samples and demonstrated good correlation (0.996) between MS/MS and Fourier-transform ion cyclotron resonance mass spectrometry results.
- Subjects :
- 0303 health sciences
Tandem
Proton
Chemistry
010401 analytical chemistry
Analytical chemistry
Protonation
General Medicine
Mass spectrometry
Tandem mass spectrometry
01 natural sciences
Atomic and Molecular Physics, and Optics
Fourier transform ion cyclotron resonance
0104 chemical sciences
Adduct
03 medical and health sciences
Spectroscopy
Ion cyclotron resonance
030304 developmental biology
Subjects
Details
- ISSN :
- 17516838 and 14690667
- Volume :
- 25
- Database :
- OpenAIRE
- Journal :
- European Journal of Mass Spectrometry
- Accession number :
- edsair.doi.dedup.....764b59211f4953925b26297d58d1c0cf