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The Effects of Magnesium and Zinc Co-Supplementation on Biomarkers of Inflammation and Oxidative Stress, and Gene Expression Related to Inflammation in Polycystic Ovary Syndrome: a Randomized Controlled Clinical Trial
- Source :
- Biological Trace Element Research. 184:300-307
- Publication Year :
- 2017
- Publisher :
- Springer Science and Business Media LLC, 2017.
-
Abstract
- Magnesium and zinc are known to exert multiple beneficial effects including anti-inflammatory and antioxidant actions. To our knowledge, data on the effects of magnesium and zinc co-supplementation on biomarkers of inflammation and oxidative stress and gene expression related to inflammation in subjects of polycystic ovary syndrome (PCOS) are scarce. This study was conducted to evaluate the effects of magnesium and zinc co-supplementation on biomarkers of inflammation and oxidative stress and gene expression related to inflammation in subjects with PCOS. This randomized double-blind, placebo-controlled trial was conducted among 60 subjects with PCOS diagnosed according to the Rotterdam criteria, aged 18–40 years old. Participants were randomly assigned into two groups to take either 250 mg of magnesium oxide plus 220 mg of zinc sulfate (containing 50 mg zinc) supplements (n = 30) or placebo (n = 30) twice a day for 12 weeks. Biomarkers of inflammation and oxidative stress were assessed at baseline and at end of treatment. Gene expression related to inflammatory cytokines was assessed in peripheral blood mononuclear cells (PBMCs) of PCOS women with RT-PCR method. After the 12-week intervention, compared with the placebo, magnesium and zinc co-supplementation significantly decreased serum high-sensitivity C-reactive protein (hs-CRP) (− 1.6 ± 2.4 vs. + 0.1 ± 0.7 mg/L, P = 0.001) and protein carbonyl (PCO) (− 0.14 ± 0.28 vs. + 0.02 ± 0.07 mmol/mg protein, P = 0.002) and significantly increased plasma total antioxidant capacity (TAC) levels (+ 60.7 ± 69.4 vs. − 1.5 ± 141.5 mmol/L, P = 0.03). Results of RT-PCR demonstrated that compared with the placebo, magnesium and zinc co-supplementation downregulated gene expression of interleukin-1 (IL-1) (P = 0.007) and tumor necrosis factor alpha (TNF-α) (P = 0.03) in PBMCs of subjects with PCOS. Overall, magnesium and zinc co-supplementation, compared with the placebo, for 12 weeks among PCOS women had beneficial effects on serum hs-CRP, plasma PCO, TAC, and gene expression of IL-1 and TNF-α. Clinical trial registration number: http://www.irct.ir: IRCT201706075623N121.
- Subjects :
- Adult
0301 basic medicine
medicine.medical_specialty
Antioxidant
Adolescent
Endocrinology, Diabetes and Metabolism
medicine.medical_treatment
Clinical Biochemistry
Gene Expression
030209 endocrinology & metabolism
Inflammation
Placebo
medicine.disease_cause
Biochemistry
Peripheral blood mononuclear cell
Antioxidants
Proinflammatory cytokine
Inorganic Chemistry
Young Adult
03 medical and health sciences
0302 clinical medicine
Double-Blind Method
Internal medicine
medicine
Humans
Magnesium
business.industry
Biochemistry (medical)
General Medicine
Polycystic ovary
Oxidative Stress
Zinc
C-Reactive Protein
030104 developmental biology
Endocrinology
Dietary Supplements
Leukocytes, Mononuclear
Cytokines
Female
Tumor necrosis factor alpha
medicine.symptom
business
Biomarkers
Oxidative stress
Polycystic Ovary Syndrome
Subjects
Details
- ISSN :
- 15590720 and 01634984
- Volume :
- 184
- Database :
- OpenAIRE
- Journal :
- Biological Trace Element Research
- Accession number :
- edsair.doi.dedup.....763e29abd3ad9706dfec5d5821bd3086