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The use of inhibitors of N-linked glycosylation and oligosaccharide processing to produce monoclonal antibodies against non-phosphorylcholine epitopes of Brugia pahangi excretory-secretory products
- Source :
- Parasitology research. 83(8)
- Publication Year :
- 1997
-
Abstract
- Adult Brugia pahangi were cultured with [3H]-choline in both the absence and the presence of either tunicamycin or 1-deoxymannojirimycin (dMM), inhibitors of N-linked glycosylation and N-linked oligosaccharide processing, respectively. Excretory-secretory products (ES) were recovered from the spent medium and examined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis/fluorography. Both inhibitors were found to prevent radiolabeling of ES, a result consistent with blockage of the addition of the highly immunodominant phosphorylcholine (PC) group. Tunicamycin/dMM-treated ES were subsequently employed to immunise a mouse in an attempt to produce monoclonal antibodies (mAbs) against non-PC epitopes of ES. Three mAbs were isolated, each of which reacted with ES but not with PC.
- Subjects :
- Glycosylation
Brugia pahangi
1-Deoxynojirimycin
medicine.drug_class
Phosphorylcholine
Antibodies, Helminth
Oligosaccharides
Monoclonal antibody
Tritium
Epitope
Choline
chemistry.chemical_compound
N-linked glycosylation
medicine
Animals
Gel electrophoresis
General Veterinary
biology
Tunicamycin
Antibodies, Monoclonal
General Medicine
biology.organism_classification
Molecular biology
Infectious Diseases
Biochemistry
chemistry
Insect Science
Antigens, Helminth
Isotope Labeling
Epitopes, B-Lymphocyte
Parasitology
Gerbillinae
Subjects
Details
- ISSN :
- 09320113
- Volume :
- 83
- Issue :
- 8
- Database :
- OpenAIRE
- Journal :
- Parasitology research
- Accession number :
- edsair.doi.dedup.....76308d826f8e89bdd464309a49bbe64a