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rhVEGF and experimental rat skin flaps: Systemic or local administration and morphological characteristics
- Source :
- Scopus-Elsevier
-
Abstract
- Skin flap survival is a significant problem in skin surgery; in particular, inadequate arterial or venous blood supply results in necrosis of the distalmost portion. The aim of this study was to evaluate the ability of Vascular Endothelial Growth Factor (VEGF) of modifying the morphological features of skin flaps. Bilateral epigastric skin flaps were raised in 16 Wistar male rats. The epigastric artery and vein of the left flaps were clamped and then injected with rhVEGF (8 rats) or saline (8 rats). The right flaps were not clamped and received rhVEGF or saline systemically. The rats were euthanized on the seventh day and flap skin samples collected. Tissue fragments were subject to immunohistochemical (rhVEGF, VEGFr, VIII factor, CD34 antibodies), ultrastructural and morphostructural investigations. The results showed that rhVEGF improved the condition of flaps and that systemic administration was effective in promoting the development of an adequate vascular network.
- Subjects :
- medicine.medical_specialty
Pathology
Necrosis
medicine.medical_treatment
030232 urology & nephrology
Biomedical Engineering
Medicine (miscellaneous)
Bioengineering
030204 cardiovascular system & hematology
Biomaterials
03 medical and health sciences
chemistry.chemical_compound
0302 clinical medicine
medicine
Vein
Saline
integumentary system
business.industry
General Medicine
Venous blood
Surgery
Vascular endothelial growth factor
Vascular endothelial growth factor A
medicine.anatomical_structure
chemistry
Systemic administration
Immunohistochemistry
medicine.symptom
business
Subjects
Details
- Database :
- OpenAIRE
- Journal :
- Scopus-Elsevier
- Accession number :
- edsair.doi.dedup.....76238a540057a19fa2571d52cd3697d9