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Biodegradation of naphthenic acids: identification of Rhodococcus opacus R7 genes as molecular markers for environmental monitoring and their application in slurry microcosms
- Publication Year :
- 2020
-
Abstract
- Nowadays, the increase of the unconventional oil deposit exploitation and the amount of oil sands process-affected waters (OSPW) in tailing ponds emerges the importance of developing bio-monitoring strategies for the restoration of these habitats. The major constituents of such deposits are naphthenic acids (NAs), emerging contaminant mixtures with toxic and recalcitrant properties. With the aim of developing bio-monitoring strategies based on culture-independent approach, we identified genes coding for enzymes involved in NA degradation from Rhodococcus opacus R7 genome, after the evaluation of its ability to mineralize model NAs. R. opacus R7 whole-genome analysis unveiled the presence of pobA and chcpca gene clusters putatively involved in NAs degradation. Gene expression analysis demonstrated the specific induction of R7 aliA1 gene, encoding for a long-chain-fatty-acid-CoA ligase, in the presence of cyclohexanecarboxylic acid (CHCA) and hexanoic acid (HA), selected as representative compounds for alicyclic and linear NAs, respectively. Therefore, aliA1 gene was selected as a molecular marker to monitor the biodegradative potential of slurry-phase sand microcosms in different conditions: spiked with CHCA, in the presence of R. opacus R7, the autochthonous microbial community, and combining these factors. Results revealed that the aliA1-targeting culture-independent approach could be a useful method for bio-monitoring of NA degradation in a model laboratory system.
- Subjects :
- Genetic Markers
Microcosm
Carboxylic Acids
Bio-monitoring
Molecular marker
Applied Microbiology and Biotechnology
03 medical and health sciences
Rhodococcus opacus
chemistry.chemical_compound
Naphthenic acid
Rhodococcus
Soil Microbiology
030304 developmental biology
Hexanoic acid
0303 health sciences
biology
030306 microbiology
Chemistry
General Medicine
Biodegradation
Cyclohexanecarboxylic acid
biology.organism_classification
Rhodococus, naphthenic acids, biomonitoring, biodegradation
Biodegradation, Environmental
Microbial population biology
Environmental chemistry
Genome, Bacterial
Water Pollutants, Chemical
Rhodococcu
Environmental Monitoring
Biotechnology
Subjects
Details
- Language :
- English
- Database :
- OpenAIRE
- Accession number :
- edsair.doi.dedup.....7611a8a3569eb4c984c56da21eb85646