Back to Search
Start Over
High-Throughput Sequencing Reveals Circulating miRNAs as Potential Biomarkers for Measuring Puberty Onset in Chicken (Gallus gallus)
- Source :
- PLoS ONE, PLoS ONE, Vol 11, Iss 5, p e0154958 (2016)
- Publication Year :
- 2016
- Publisher :
- Public Library of Science, 2016.
-
Abstract
- There are still no highly sensitive and unique biomarkers for measurement of puberty onset. Circulating miRNAs have been shown to be promising biomarkers for diagnosis of various diseases. To identify circulating miRNAs that could be served as biomarkers for measuring chicken (Gallus gallus) puberty onset, the Solexa deep sequencing was performed to analyze the miRNA expression profiles in serum and plasma of hens from two different pubertal stages, before puberty onset (BO) and after puberty onset (AO). 197 conserved and 19 novel miRNAs (reads > 10) were identified as serum/plasma-expressed miRNAs in the chicken. The common miRNA amounts and their expression changes from BO to AO between serum and plasma were very similar, indicating the different treatments to generate serum and plasma had quite small influence on the miRNAs. 130 conserved serum-miRNAs were showed to be differentially expressed (reads > 10, P < 0.05) from BO to AO, with 68 up-regulated and 62 down-regulated. 4829 putative genes were predicted as the targets of the 40 most differentially expressed miRNAs (|log2(fold-change)|>1.0, P < 0.01). Functional analysis revealed several pathways that were associated with puberty onset. Further quantitative real-time PCR (RT-qPCR) test found that a seven-miRNA panel, including miR-29c, miR-375, miR-215, miR-217, miR-19b, miR-133a and let-7a, had great potentials to serve as novel biomarkers for measuring puberty onset in chicken. Due to highly conserved nature of miRNAs, the findings could provide cues for measurement of puberty onset in other animals as well as humans.
- Subjects :
- 0301 basic medicine
Physiology
Molecular biology
lcsh:Medicine
Biochemistry
Poultry
Endocrinology
Sequencing techniques
Blood plasma
Medicine and Health Sciences
Sexual maturity
Gamefowl
Sexual Maturation
lcsh:Science
Multidisciplinary
High-Throughput Nucleotide Sequencing
Agriculture
RNA sequencing
Animal Models
Hematology
Body Fluids
Up-Regulation
Nucleic acids
Blood
Vertebrates
RNA extraction
Anatomy
Research Article
medicine.medical_specialty
Livestock
Down-Regulation
Biology
DNA sequencing
Deep sequencing
Blood Plasma
Birds
03 medical and health sciences
Model Organisms
Extraction techniques
Downregulation and upregulation
Internal medicine
microRNA
medicine
Genetics
Animals
Non-coding RNA
Biology and life sciences
Endocrine Physiology
Gene Expression Profiling
lcsh:R
Puberty
Organisms
Gene regulation
Chicken Models
Gene expression profiling
Research and analysis methods
MicroRNAs
030104 developmental biology
Molecular biology techniques
Fowl
Amniotes
RNA
lcsh:Q
Gene expression
Chickens
Biomarkers
Subjects
Details
- Language :
- English
- ISSN :
- 19326203
- Volume :
- 11
- Issue :
- 5
- Database :
- OpenAIRE
- Journal :
- PLoS ONE
- Accession number :
- edsair.doi.dedup.....760cd547a46c34841e59b78e2228f55d