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The Role of Bone Marrow-Derived Adult Stem Cells in a Transgenic Mouse Model of Allergic Asthma
- Source :
- Respiration. 83:74-80
- Publication Year :
- 2011
- Publisher :
- S. Karger AG, 2011.
-
Abstract
- Background: Asthmatic airway remodeling is an abnormal injury/repair process of the small airways caused by chronic inflammation in which the quantities of multiple cells increase dramatically. However, the origin of these proliferative cells is still undetermined. Objective: The aim of this study was to examine whether bone marrow (BM)-derived adult stem cells are responsible for the proliferative cells in asthmatic airway remodeling. Methods: Adult mice were durably engrafted with BM isolated from green fluorescent protein (GFP) transgenic mice. Using GFP BM chimera mice, an ovalbumin (OVA)-induced chronic asthma mouse model was established. The distribution of BM-derived GFP+ cells in the lungs of chronic asthma mice was detected by fluorescence microscopy. The phenotype of BM-derived GFP+ cells in the lung tissues of chronic asthma mice was analyzed by flow cytometry. Results: BM chimera mice were successfully generated, with no detectable radioactive inflammation observed. Using BM chimera mice, we established a mouse model of chronic asthma characterized by a significant increase in the thickness of the airway subepithelial basement membrane and smooth muscle layers. OVA treatment caused many GFP+ cells to appear at sites of small airway inflammation. The extravascular localization of some GFP+ cells and their morphology were not consistent with leukocytes. Flow cytometric analysis of lung cells revealed a significant increase in type I collagen (Col I)+GFP+ cells and α-smooth muscle actin (α-SMA)+GFP+ cells in OVA-treated GFP BM chimera mice. Conclusions: Considerable numbers of Col I- and α-SMA-producing cells originated from BM in the lung tissues of mice with OVA-induced chronic asthma.
- Subjects :
- Pulmonary and Respiratory Medicine
Genetically modified mouse
Pathology
medicine.medical_specialty
Cellular differentiation
Bone Marrow Cells
Mice, Transgenic
Inflammation
Mice
Immunity
Hypersensitivity
medicine
Animals
Asthma
Immunity, Cellular
business.industry
Cell Differentiation
respiratory system
Flow Cytometry
Hematopoietic Stem Cells
medicine.disease
respiratory tract diseases
Mice, Inbred C57BL
Disease Models, Animal
medicine.anatomical_structure
Asthmatic Airway Remodeling
Immunology
Airway Remodeling
Female
Bone marrow
medicine.symptom
business
Bronchoalveolar Lavage Fluid
Adult stem cell
Subjects
Details
- ISSN :
- 14230356 and 00257931
- Volume :
- 83
- Database :
- OpenAIRE
- Journal :
- Respiration
- Accession number :
- edsair.doi.dedup.....760a589ae6df2f4aaa227c3fa3e23715