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Delay in maternal transcript degradation in ovine embryos derived from low competence oocytes
- Source :
- Molecular reproduction and development. 85(5)
- Publication Year :
- 2017
-
Abstract
- Oocytes from prepubertal animals have a reduced ability to undergo embryo development and produce viable offspring. The present work used an ovine model consisting of oocytes derived from adult and prepubertal donors to assess the molecular status of oocytes and preimplantation embryos with different developmental competence. The lower potential of oocytes of young donors was confirmed in terms of in vitro developmental capabilities and kinetics. A panel of genes including maternal effect (DPPA3, GDF9, NMP2, ZAR1) and housekeeping genes (ACTB, RPL19, SDHA, YWHAZ, ATP1A1), genes involved in DNA methylation (DNMT1, DNMT3A, DNMT3B), genomic imprinting (IGF2R), pluripotency (NANOG, POU5F1) and cell cycle regulation (CCNB1, CDK1, MELK) was relatively quantified. Temporal analysis during oocyte maturation and preimplantation embryo development evidenced patterns associated with donor age. With a few gene-specific exceptions, the differential model showed a reduced transcript abundance in immature prepubertal oocytes that completely reversed trend after fertilization, when higher mRNA levels were consistently observed in early embryos, indicating a delay in maternal transcript degradation. We propose that the molecular shortage in the prepubertal oocyte may affect its developmental potential and impair the early pathways of maternal mRNA clearance in the embryo. While confirming the different potential of oocytes derived from adult and prepubertal donors, our work showed for the first time a consistent delay in maternal transcript degradation in embryos derived from low competence oocytes that interestingly recalls the delayed developmental kinetics. Such abnormal transcript persistence may hinder further development and represents a novel perspective on the complexity of developmental competence.
- Subjects :
- 0301 basic medicine
Embryonic Development
Biology
Andrology
03 medical and health sciences
Genomic Imprinting
Pregnancy
Genetics
medicine
Animals
DNA (Cytosine-5-)-Methyltransferases
Sheep
Embryogenesis
Maternal effect
Gene Expression Regulation, Developmental
Embryo
Cell Biology
DNA Methylation
Oocyte
Housekeeping gene
030104 developmental biology
medicine.anatomical_structure
Blastocyst
embryonic structures
DNA methylation
YWHAZ
Oocytes
Female
Genomic imprinting
Developmental Biology
Subjects
Details
- ISSN :
- 10982795
- Volume :
- 85
- Issue :
- 5
- Database :
- OpenAIRE
- Journal :
- Molecular reproduction and development
- Accession number :
- edsair.doi.dedup.....7601b3cb788aa77619d34d35df514f66