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Prevalence of carriage of Shiga toxin-producing Escherichia coli serotypes O157:H7, O26:H11, O103:H2, O111:H8, and O145:H28 among slaughtered adult cattle in France
- Source :
- Applied and Environmental Microbiology, Applied and Environmental Microbiology, American Society for Microbiology, 2015, 81 (4), pp.1397-1405. ⟨10.1128/AEM.03315-14⟩
- Publication Year :
- 2014
-
Abstract
- The main pathogenic enterohemorrhagic Escherichia coli (EHEC) strains are defined as Shiga toxin (Stx)-producing E. coli (STEC) belonging to one of the following serotypes: O157:H7, O26:H11, O103:H2, O111:H8, and O145:H28. Each of these five serotypes is known to be associated with a specific subtype of the intimin-encoding gene ( eae ). The objective of this study was to evaluate the prevalence of bovine carriers of these “top five” STEC in the four adult cattle categories slaughtered in France. Fecal samples were collected from 1,318 cattle, including 291 young dairy bulls, 296 young beef bulls, 337 dairy cows, and 394 beef cows. A total of 96 E. coli isolates, including 33 top five STEC and 63 atypical enteropathogenic E. coli (aEPEC) isolates, with the same genetic characteristics as the top five STEC strains except that they lacked an stx gene, were recovered from these samples. O157:H7 was the most frequently isolated STEC serotype. The prevalence of top five STEC (all serotypes included) was 4.5% in young dairy bulls, 2.4% in young beef bulls, 1.8% in dairy cows, and 1.0% in beef cows. It was significantly higher in young dairy bulls ( P < 0.05) than in the other 3 categories. The basis for these differences between categories remains to be elucidated. Moreover, simultaneous carriage of STEC O26:H11 and STEC O103:H2 was detected in one young dairy bull. Lastly, the prevalence of bovine carriers of the top five STEC, evaluated through a weighted arithmetic mean of the prevalence by categories, was estimated to 1.8% in slaughtered adult cattle in France.
- Subjects :
- Serotype
Male
Disease reservoir
genome sequence
intimin gene
[SDV]Life Sciences [q-bio]
animal diseases
medicine.disease_cause
Shiga Toxins
Applied Microbiology and Biotechnology
serogroups O26
fluids and secretions
Prevalence
beef-cattle
time
2. Zero hunger
0303 health sciences
Ecology
biology
Shiga-Toxigenic Escherichia coli
Public and Environmental Health Microbiology
Escherichia coli Proteins
Shiga toxin
immunomagnetic separation
Carrier State
Female
hemolytic-uremic syndrome
France
Abattoirs
Biotechnology
Meat
O157-H7
Microbiology
5'-nuclease PCR
03 medical and health sciences
medicine
Animals
Escherichia coli
Shiga toxin-producing Escherichia coli
Feces
030304 developmental biology
Disease Reservoirs
030306 microbiology
E. coli
biochemical phenomena, metabolism, and nutrition
bacterial infections and mycoses
Carriage
Asymptomatic Diseases
biology.protein
bacteria
Cattle
Dairy bull
Food Science
Subjects
Details
- ISSN :
- 10985336 and 00992240
- Volume :
- 81
- Issue :
- 4
- Database :
- OpenAIRE
- Journal :
- Applied and environmental microbiology
- Accession number :
- edsair.doi.dedup.....75c9581994aea6253c26341efd837207