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EXECUTER1- and EXECUTER2-dependent transfer of stress-related signals from the plastid to the nucleus of Arabidopsis thaliana
- Source :
- Proceedings of the National Academy of Sciences. 104:10270-10275
- Publication Year :
- 2007
- Publisher :
- Proceedings of the National Academy of Sciences, 2007.
-
Abstract
- Shortly after the release of singlet oxygen ( 1 O 2 ), drastic changes in nuclear gene expression occur in the conditional flu mutant of Arabidopsis that reveal a rapid transfer of signals from the plastid to the nucleus. In contrast to retrograde control of nuclear gene expression by plastid signals described earlier, the primary effect of 1 O 2 generation in the flu mutant is not the control of chloroplast biogenesis but the activation of a broad range of signaling pathways known to be involved in biotic and abiotic stress responses. This activity of a plastid-derived signal suggests a new function of the chloroplast, namely that of a sensor of environmental changes that activates a broad range of stress responses. Inactivation of the plastid protein EXECUTER1 attenuates the extent of 1 O 2 -induced up-regulation of nuclear gene expression, but it does not fully eliminate these changes. A second related nuclear-encoded protein, dubbed EXECUTER2, has been identified that is also implicated with the signaling of 1 O 2 -dependent nuclear gene expression changes. Like EXECUTER1, EXECUTER2 is confined to the plastid. Inactivation of both EXECUTER proteins in the ex1 / ex2 / flu triple mutant is sufficient to suppress the up-regulation of almost all 1 O 2 -responsive genes. Retrograde control of 1 O 2 -responsive genes requires the concerted action of both EXECUTER proteins within the plastid compartment.
- Subjects :
- DNA, Complementary
Nuclear gene
Recombinant Fusion Proteins
Green Fluorescent Proteins
Molecular Sequence Data
Mutant
Arabidopsis
Genes, Plant
Protochlorophyllide
Gene Expression Regulation, Plant
medicine
Arabidopsis thaliana
Amino Acid Sequence
Plastids
Plastid
Alleles
Oligonucleotide Array Sequence Analysis
Cell Nucleus
Genetics
Regulation of gene expression
Multidisciplinary
Sequence Homology, Amino Acid
Singlet Oxygen
biology
Arabidopsis Proteins
Reverse Transcriptase Polymerase Chain Reaction
Homozygote
food and beverages
Biological Sciences
biology.organism_classification
Cell biology
Oxidative Stress
Cell nucleus
medicine.anatomical_structure
Amino Acid Substitution
Signal transduction
Signal Transduction
Subjects
Details
- ISSN :
- 10916490 and 00278424
- Volume :
- 104
- Database :
- OpenAIRE
- Journal :
- Proceedings of the National Academy of Sciences
- Accession number :
- edsair.doi.dedup.....75a6fd3e225a308e190812a9e93bf4b5