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Cooperative interaction between metallosurfactants, derived from the [Ru(2,2'-bpy)3](2+) complex, and DNA
- Source :
- Colloids and surfaces. B, Biointerfaces. 135
- Publication Year :
- 2015
-
Abstract
- With the idea of improving and advancing the design and preparation of new reagents based on cationic surfactants for gene therapy, two luminescent metallosurfactants derived from the [Ru(2,2'-bpy)3](2+) complex were synthesized. Their interaction with DNA and the effect they exert on the conformation of the polynucleotide were studied by using different techniques. The equilibrium binding constants, Kb, of the two surfactants to DNA were obtained at different molar ratios X=[surfactant]/[DNA]. The observed sigmoidal dependence of Kb on X confirms the cooperative character of the binding. After the addition of a determined surfactant concentration, the condensation of the polymer was observed. The amount of surfactant needed to produce this conformational change is lower for the double stranded surfactant than for the single chain surfactant due to a stronger hydrophobic interaction. The addition of α-cyclodextrin molecules to the metallosurfactant/DNA solutions results in polynucleotide decompaction, which confirms the importance of the hydrophobic interactions in the condensation of the polynucleotide. Results also show the importance of choosing both a proper system to study and the most seeming measuring technique to use. It is demonstrated that, in some cases, the use of several techniques is desirable to obtain reliable and accurate results.
- Subjects :
- Conformational change
Stereochemistry
Cationic polymerization
Surfaces and Interfaces
General Medicine
DNA Solutions
DNA
Rubidium
Hydrophobic effect
chemistry.chemical_compound
Crystallography
Surface-Active Agents
Colloid and Surface Chemistry
Spectrometry, Fluorescence
Pulmonary surfactant
chemistry
Polynucleotide
Metals
Molecule
Physical and Theoretical Chemistry
Biotechnology
Subjects
Details
- ISSN :
- 18734367
- Volume :
- 135
- Database :
- OpenAIRE
- Journal :
- Colloids and surfaces. B, Biointerfaces
- Accession number :
- edsair.doi.dedup.....759a5d12fb6838b5e870ef0fe995f39f