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Vascular endothelial growth factor induces anti-Müllerian hormone receptor 2 overexpression in ovarian granulosa cells of in vitro fertilization/intracytoplasmic sperm injection patients
- Source :
- Molecular Medicine Reports. 13:5157-5162
- Publication Year :
- 2016
- Publisher :
- Spandidos Publications, 2016.
-
Abstract
- Misregulation of vascular endothelial growth factor A (VEGF‑A) has been implicated in numerous types of ovarian disease, such as polycystic ovarian syndrome, ovarian hyperstimulation syndrome, endometriosis and ovarian cancer. VEGF regulates blood vessel permeability and angiogenesis. In our previous study, VEGF‑regulated gene expression was profiled in the uterus of a transgenic mouse model with repressed VEGF expression, which indicated that VEGF is an important regulator in controlling gene expression in the uterus. The anti‑Müllerian hormone (AMH) is expressed by ovarian granulosa cells (GCs) and acts through its type 2 receptor, AMH receptor 2 (AMHR2). Serum AMH levels are used to predict ovarian reserves and the small antral follicles contribute markedly to the serum AMH level. AMH recruits primordial follicles and inhibits excessive follicular development by follicular stimulating hormone (FSH). However, AMH may be influenced by suppression of gonadotrophin secretion and VEGF inhibition. In the current study, human primary ovarian GCs were isolated from ovarian follicle fluid of in vitro fertilization/intracytoplasmic sperm injection cycles (IVF/ICSI). It was identified that the FSH receptor was consistently expressed in the isolated cells. VEGF‑A treatment stimulated AMHR2 overexpression at the gene and protein levels. In addition, VEGF induced AMHR2 expression on the surface of the isolated GCs from mature follicles. The VEGF treatment was also performed in an ovarian granulosa‑like cell line, KGN. AMH and AMHR2 are co‑expressed in normal GCs; however, as a result of VEGF misregulation, AMHR2 overexpression increases AMH binding, which may attenuate follicular or oocyte maturation. However, the associated function and underlying mechanism requires further investigation.
- Subjects :
- Adult
Male
Vascular Endothelial Growth Factor A
0301 basic medicine
endocrine system
Cancer Research
medicine.medical_specialty
Receptors, Peptide
endocrine system diseases
Ovarian hyperstimulation syndrome
Biology
Biochemistry
Cell Line
Ovarian disease
Mice
03 medical and health sciences
chemistry.chemical_compound
0302 clinical medicine
Internal medicine
Follicular phase
Anti-Müllerian hormone receptor
Genetics
medicine
Animals
Humans
Sperm Injections, Intracytoplasmic
Molecular Biology
Granulosa Cells
030219 obstetrics & reproductive medicine
urogenital system
Antral follicle
medicine.disease
female genital diseases and pregnancy complications
Vascular endothelial growth factor
030104 developmental biology
Endocrinology
Gene Expression Regulation
Oncology
chemistry
Molecular Medicine
Female
Ovarian cancer
Follicle-stimulating hormone receptor
Receptors, Transforming Growth Factor beta
hormones, hormone substitutes, and hormone antagonists
Subjects
Details
- ISSN :
- 17913004 and 17912997
- Volume :
- 13
- Database :
- OpenAIRE
- Journal :
- Molecular Medicine Reports
- Accession number :
- edsair.doi.dedup.....7598ec1524368dc273b16263449bc9e3