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Anxiolytic-like effect of chalcone N-{4’[(2E)-3-(3-nitrophenyl)-1-(phenyl)prop-2-en-1-one]} acetamide on adult zebrafish (Danio rerio): Involvement of the 5-HT system
- Source :
- Biochemical and Biophysical Research Communications. 526:505-511
- Publication Year :
- 2020
- Publisher :
- Elsevier BV, 2020.
-
Abstract
- The action of anxiolytic compounds that act on selective serotonin receptors (SSRIs) have been scarcely evaluated. Serotonergic drugs have been shown to be effective in treating anxiety without presenting adverse effects as benzodiazepines. However, the anxiolytic effects take days to occur. This study aimed to evaluate the anxiolytic effect of the synthetic chalcone, 4’-[(2E) -3- (3-nitrophenyl) -1- (phenyl) prop-2-en-1-one] acetamide (PAAMNBA), and its possible mechanism of action in adult zebrafish (Danio rerio). PAAMNBA was synthesized with a yield of 51.3% and its chemical structure was determined by 1H and 13C NMR. Initially, PAAPMNBA was intraperitoneally administered to zebrafish (n = 6/group) at doses of 4, 12, or 40 mg/kg, and the animals were subsequently subjected to acute and open field toxicity tests. PAAMNBA was administered to the other groups (n = 6/group) for analyzing its effect in the light and dark test. The involvement of the serotonergic (5HT) system was also evaluated using 5-HTR 1, 5-HTR 2A/2C, and 5-HTR 3A/3B receptor antagonists, namely, pizotifeo, granizetron, and ciproeptadina, respectively. Molecular coupling was performed using the 5-HT1 receptor. PAAMNBA was found to be non-toxic, reduced the locomotor activity, and had an anxiolytic effect in adult zebrafish. The effect was reduced by pretreatment with pizotifene and was not reversed by treatment with granizetron and cyproeptadine. A previous in vivo molecular coupling study indicated that chalcones interact with the 5-HT1 receptor. The results suggested that the chalcone, PAAPMNBA, has anxiolytic activity, that is mediated by the serotonergic system via the 5-HT1 receptor. The interaction of PAAPMNBA with the 5-HT1 receptor was confirmed by molecular docking studies.
- Subjects :
- 0301 basic medicine
Serotonin
Chalcone
medicine.drug_class
Biophysics
Danio
Anxiety
Pharmacology
Serotonergic
Biochemistry
Anxiolytic
Open field
03 medical and health sciences
chemistry.chemical_compound
0302 clinical medicine
Acetamides
Drug Discovery
medicine
Animals
Receptor
Molecular Biology
Zebrafish
5-HT receptor
biology
Cell Biology
Zebrafish Proteins
biology.organism_classification
Molecular Docking Simulation
030104 developmental biology
Anti-Anxiety Agents
Mechanism of action
chemistry
030220 oncology & carcinogenesis
medicine.symptom
Receptors, Serotonin, 5-HT1
Locomotion
Subjects
Details
- ISSN :
- 0006291X
- Volume :
- 526
- Database :
- OpenAIRE
- Journal :
- Biochemical and Biophysical Research Communications
- Accession number :
- edsair.doi.dedup.....7593568f19b98ea5eea639ff06c94468