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Identification and isolation of lectin nucleotide sequences and species relationships in the genus Lens Miller
- Source :
- Theoretical and applied genetics 108 (2004): 1098–1102. doi:10.1007/s11032-009-9260-4, info:cnr-pdr/source/autori:Galasso I., Lioi L., Lanave C., Bollini R., Sparvoli F./titolo:Identification and isolation of lectin nucleotide sequences and species relationships in the genus Lens (Miller)/doi:10.1007%2Fs11032-009-9260-4/rivista:Theoretical and applied genetics/anno:2004/pagina_da:1098/pagina_a:1102/intervallo_pagine:1098–1102/volume:108
- Publication Year :
- 2003
-
Abstract
- Genes for lectin, a component of legume storage proteins, were identified and characterised in two lentil cultivars ( Lens culinaris ssp. culinaris) and six wild relatives. In each taxon no differences were found among the two or three lectin clones sequenced, while differences were observed among lectin genes isolated from the different taxa. All of the clones analysed contained an insert of 828 bp and showed a high similarity with the nucleotide sequence of Pisum sativum seed lectin, PSL1. The deduced amino acid lectin sequences in all taxa were 275 amino acids long, and their multiple alignment showed that most of the variation among them occurred in regions which are not important for metal- and sugar-binding. The data from Southern blot analysis indicated the presence of only one lectin gene in all Lens taxa except L. tomentosus. Phylogenetic analyses carried out on the lectin sequences showed the existence of two main clusters and clearly indicated that L. nigricans falls outside the two groups.
- Subjects :
- Molecular Sequence Data
two-chain lectin
Biology
Pisum
Genetics
Storage protein
Cluster Analysis
Gene
Phylogeny
Southern blot
DNA Primers
chemistry.chemical_classification
Multiple sequence alignment
Phylogenetic tree
Base Sequence
Models, Genetic
Nucleic acid sequence
wild Lens species
Lectin
food and beverages
General Medicine
Sequence Analysis, DNA
biology.organism_classification
Blotting, Southern
chemistry
biology.protein
Electrophoresis, Polyacrylamide Gel
Lens Plant
Plant Lectins
Agronomy and Crop Science
Biotechnology
Subjects
Details
- ISSN :
- 00405752
- Volume :
- 108
- Issue :
- 6
- Database :
- OpenAIRE
- Journal :
- TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik
- Accession number :
- edsair.doi.dedup.....756fceecdc3a79c304867b5d04cc6d2c