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Asynchronous fate decisions by single cells collectively ensure consistent lineage composition in the mouse blastocyst
- Source :
- Nature Communications, Vol 7, Iss 1, Pp 1-14 (2016), Nature Communications
- Publication Year :
- 2016
- Publisher :
- Nature Portfolio, 2016.
-
Abstract
- Intercellular communication is essential to coordinate the behaviour of individual cells during organismal development. The preimplantation mammalian embryo is a paradigm of tissue self-organization and regulative development; however, the cellular basis of these regulative abilities has not been established. Here we use a quantitative image analysis pipeline to undertake a high-resolution, single-cell level analysis of lineage specification in the inner cell mass (ICM) of the mouse blastocyst. We show that a consistent ratio of epiblast and primitive endoderm lineages is achieved through incremental allocation of cells from a common progenitor pool, and that the lineage composition of the ICM is conserved regardless of its size. Furthermore, timed modulation of the FGF-MAPK pathway shows that individual progenitors commit to either fate asynchronously during blastocyst development. These data indicate that such incremental lineage allocation provides the basis for a tissue size control mechanism that ensures the generation of lineages of appropriate size.<br />Early embryonic cell fate and lineage specification is tightly regulated in the preimplantation mammalian embryo. Here, the authors quantitatively examine the ratio of epiblast to primitive endoderm lineages in the blastocyst and show composition of the inner cell mass is conserved, independent of its size.
- Subjects :
- 0301 basic medicine
Lineage (genetic)
animal structures
Cellular differentiation
Science
Embryonic Development
General Physics and Astronomy
Germ layer
Biology
Article
General Biochemistry, Genetics and Molecular Biology
Embryo Culture Techniques
Mice
03 medical and health sciences
medicine
Animals
Inner cell mass
Cell Lineage
Blastocyst
Progenitor cell
reproductive and urinary physiology
Genetics
Multidisciplinary
Embryogenesis
Cell Differentiation
General Chemistry
Cell biology
030104 developmental biology
medicine.anatomical_structure
Epiblast
embryonic structures
Germ Layers
Signal Transduction
Subjects
Details
- Language :
- English
- ISSN :
- 20411723
- Volume :
- 7
- Issue :
- 1
- Database :
- OpenAIRE
- Journal :
- Nature Communications
- Accession number :
- edsair.doi.dedup.....755ff6b5f7bc3ce3079da8be90eadcbd